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Literature summary for 2.1.2.2 extracted from
- A rapid screen of active site mutants in glycinamide ribonucleotide transformylase (1996), Biochemistry, 35, 8855-8862.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| second GAR transformylase encoded by the Escherichia coli purT gene | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
10-formyl-5,8-dideazafolate | mutant H108A | Escherichia coli |  |
| 0.017 | - |
10-formyl-5,8-dideazafolate | - |
Escherichia coli | |
| 0.018 | - |
beta-glycinamide ribonucleotide | mutant H108Q | Escherichia coli | |
| 0.019 | - |
beta-glycinamide ribonucleotide | - |
Escherichia coli | |
| 0.024 | - |
10-formyl-5,8-dideazafolate | mutant H108Q | Escherichia coli | |
| 0.025 | - |
10-formyl-5,8-dideazafolate | mutant D144A | Escherichia coli | |
| 0.029 | - |
beta-glycinamide ribonucleotide | mutant D144A | Escherichia coli | |
| 0.054 | - |
beta-glycinamide ribonucleotide | mutant H108A | Escherichia coli | |
| 0.9 | - |
10-formyl-5,8-dideazafolate | mutant N106G | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23240 | - |
- |
Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | Bacillus subtilis | - |
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | Saccharomyces cerevisiae | - |
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | Gallus gallus | essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | Drosophila melanogaster | essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | Escherichia coli | essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | Homo sapiens | essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Drosophila melanogaster | - |
fruit fly | - |
| Escherichia coli | - |
strains DH5alpha, BL21(DE3), TX680 | - |
| Gallus gallus | - |
chicken | - |
| Homo sapiens | - |
human | - |
| Saccharomyces cerevisiae | - |
yeast | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | - |
Gallus gallus | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | - |
Drosophila melanogaster | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | - |
Bacillus subtilis | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | - |
Escherichia coli | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | - |
Homo sapiens | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | - |
Saccharomyces cerevisiae | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway | Gallus gallus | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway | Drosophila melanogaster | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway | Escherichia coli | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway | Homo sapiens | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Gallus gallus |
| monomer | - |
Bacillus subtilis |
| monomer | - |
Escherichia coli |
| monomer | - |
Homo sapiens |
| monomer | - |
Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| purN transformylase | - |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 10-formyl-5,8-dideazafolate | - |
Escherichia coli | |
| formate | second GAR transformylase using formate and ATP in place of 10-formyltetrahydrofolate | Escherichia coli | |
| N10-formyltetrahydrofolate | - |
Gallus gallus | |
| N10-formyltetrahydrofolate | - |
Escherichia coli | |
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