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Literature summary for 2.1.2.10 extracted from
- Glycine metabolism by rat liver mitochondria. Reconstruction of the reversible glycine cleavage system with partially purified protein components (1974), Arch. Biochem. Biophys., 164, 624-633.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetamide | 5 mM, 70% inhibition | Rattus norvegicus |  |
| N-ethylmaleimide | 0.1 mM, 70% inhibition | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrial inner membrane | - |
Rattus norvegicus | 5743 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate | Rattus norvegicus | glycine metabolism | dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
male Wistar rats | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 24 h, 60% loss of activity | Rattus norvegicus |
| 0-4°C, 1 week, stable | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | enzyme is a component of the reversible glycine cleavage system which is composed of 4 protein components named as P-, H-, L- and T-protein | Rattus norvegicus | ? | - |
? | |
| S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate | decarboxylated glycine moiety attached to H-protein + tetrahydrofolate as substrates | Rattus norvegicus | dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3 | - |
r | |
| S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate | T-protein participates in the formation of the one carbon unit and ammonia or the reverse reaction | Rattus norvegicus | dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3 | - |
r | |
| S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate | glycine metabolism | Rattus norvegicus | dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
1 min, 20% loss of activity | Rattus norvegicus |
| 70 | - |
1 min, complete loss of activity | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| tetrahydrofolate | - |
Rattus norvegicus | |
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Release 2023.1 (January 2023)
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