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Literature summary for 2.1.2.1 extracted from

  • Schweitzer, J.E.; Stolz, M.; Diesveld, R.; Etterich, H.; Eggeling, L.
    The serine hydroxymethyltransferase gene glyA in Corynebacterium glutamicum is controlled by GlyR (2009), J. Biotechnol., 139, 214-221.
    View publication on PubMed
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Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum
-
 strain ATCC13032
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Corynebacterium glutamicum ATCC 13032
-
 strain ATCC13032
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0385
-
 in the presence of glycine, in 200 mM HEPES buffer, pH 7.0 Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine + tetrahydrofolate
-
 Corynebacterium glutamicum glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?
L-serine + tetrahydrofolate
-
 Corynebacterium glutamicum ATCC 13032 glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?

Synonyms

Synonyms Comment Organism
serine hydroxymethyltransferase
-
 Corynebacterium glutamicum
SHMT
-
 Corynebacterium glutamicum

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Corynebacterium glutamicum

Expression

Organism Comment Expression
Corynebacterium glutamicum about 3fold increase of SHMT activity during exponential growth with a further increase at the onset of the stationary phase, the essential SHMT has highest activity in the stationary phase and the regulator GlyR acts as an activator of transcription in this growth phase. Addition of glycine results in a slight but significant increase of SHMTactivity by 75% up