Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Hyphomicrobium methylovorum | |
2,2'-dipyridyl | - |
Hyphomicrobium methylovorum | |
5,5-dithiobis(2-nitrobenzoic acid) | - |
Hyphomicrobium methylovorum | |
Ag+ | strong | Hyphomicrobium methylovorum | |
Cu2+ | strong | Hyphomicrobium methylovorum | |
D-cycloserine | - |
Hyphomicrobium methylovorum | |
Hg2+ | strong | Hyphomicrobium methylovorum | |
KCl | - |
Hyphomicrobium methylovorum | |
KCN | - |
Hyphomicrobium methylovorum | |
LiCl | - |
Hyphomicrobium methylovorum | |
additional information | not: EDTA | Hyphomicrobium methylovorum | |
NaCl | - |
Hyphomicrobium methylovorum | |
NaF | - |
Hyphomicrobium methylovorum | |
NH2OH | - |
Hyphomicrobium methylovorum | |
Pb2+ | strong | Hyphomicrobium methylovorum | |
PCMB | strong | Hyphomicrobium methylovorum | |
Penicillamine | - |
Hyphomicrobium methylovorum | |
phenylhydrazine | partially reversible by pyridoxal 5'-phosphate | Hyphomicrobium methylovorum | |
Semicarbazide | - |
Hyphomicrobium methylovorum | |
Sodium borohydride | - |
Hyphomicrobium methylovorum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Hyphomicrobium methylovorum | |
0.04 | 0.046 | tetrahydrofolate | glycine | Hyphomicrobium methylovorum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
2 * 48000, SDS-PAGE | Hyphomicrobium methylovorum |
80000 | - |
HPLC gel filtration | Hyphomicrobium methylovorum |
98000 | - |
gel filtration | Hyphomicrobium methylovorum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + tetrahydrofolate | Hyphomicrobium methylovorum | key enzyme of serine pathway for assimilation of C1-compounds | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hyphomicrobium methylovorum | - |
strain GM2, a glycine resistant mutant derived from strain KM146 | - |
Purification (Comment) | Organism |
---|---|
- |
Hyphomicrobium methylovorum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.9 | - |
serine synthesis | Hyphomicrobium methylovorum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + tetrahydrofolate | - |
Hyphomicrobium methylovorum | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
r | |
L-serine + tetrahydrofolate | key enzyme of serine pathway for assimilation of C1-compounds | Hyphomicrobium methylovorum | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 48000, SDS-PAGE | Hyphomicrobium methylovorum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
serine synthesis | Hyphomicrobium methylovorum |
37 | 40 | 3-phenylserine degradation | Hyphomicrobium methylovorum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
29 | 42 | serine synthesis, about half-maximal activity at 28.5°C and 90% of maximal activity at 42°C | Hyphomicrobium methylovorum |
30 | 42 | 2-phenylserine degradation, about half-maximal activity at 29.5°C and 80% of maximal activity at 42°C | Hyphomicrobium methylovorum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
below, at least 10 min stable | Hyphomicrobium methylovorum |
45 | - |
above, rapid loss of activity | Hyphomicrobium methylovorum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Hyphomicrobium methylovorum |
7.5 | 8 | 3-phenylserine degradation | Hyphomicrobium methylovorum |
8 | - |
serine synthesis | Hyphomicrobium methylovorum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | serine synthesis: about 80% of maximal activity at pH 7.0 and 9.0 | Hyphomicrobium methylovorum |
7.2 | 8.6 | 3-phenylserine degradation: about half-maximal activity at pH 7.2 and 8.6 | Hyphomicrobium methylovorum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | stable | Hyphomicrobium methylovorum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | 2 mol per mol enzyme | Hyphomicrobium methylovorum | |
tetrahydrofolate | requirement with L-serine or L-2-methylserine as substrate | Hyphomicrobium methylovorum |