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Literature summary for 2.1.1.B76 extracted from

  • Joe, E.J.; Kim, B.G.; An, B.C.; Chong, Y.; Ahn, J.H.
    Engineering of flavonoid O-methyltransferase for a novel regioselectivity (2010), Mol. Cells, 30, 137-141.
    View publication on PubMed
Search for more literature for 2.1.1.B76

Cloned(Commentary)

Cloned (Comment) Organism
-
 Populus sp.
gene POMT7, error-prone polymerase chain reaction, expression of GST-tagged wild-type POMT7 and mutant POMT7-M1 in Escherichia coli Populus sp.

Protein Variants

Protein Variants Comment Organism
D257G random mutagenesis, the mutant of POMT-7, POMT7-M1, has a novel regioselectivity, from large scale mutant screening, mutant D257G methylates the 3-hydroxyl group of flavonols in addition to 7-hydrdoxyl group Populus sp.
N257G the mutant methylates the 3-hydroxyl group of flavonols in addition to 7-hydrdoxyl group. The wild type POMT7 exhibits better activity toward kaempferol and quercetin than the mutant enzyme Populus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0298
-
 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4H-1-benzopyran-4-one i.e. quercetin, pH 7.5, 37°C, wild-type enzyme Populus sp.
0.0298
-
 kaempferol pH 7.5, 37°C, recombinant wild-type POMT7 Populus sp.
0.0324
-
 3,4',5,7-tetrahydroxyflavanone i.e. kaempferol, pH 7.5, 37°C, wild-type enzyme Populus sp.
0.0324
-
 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4H-1-benzopyran-4-one i.e. quercetin, pH 7.5, 37°C, mutant enzyme N257G Populus sp.
0.0324
-
 kaempferol pH 7.5, 37°C, recombinant mutant POMT7-M1 Populus sp.
0.0324
-
 quercetin pH 7.5, 37°C, recombinant wild-type POMT7 Populus sp.
0.0378
-
 3,4',5,7-tetrahydroxyflavanone i.e. kaempferol, pH 7.5, 37°C, mutant enzyme N257G Populus sp.
0.0378
-
 quercetin pH 7.5, 37°C, recombinant mutant POMT7-M1 Populus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + kaempferol Populus sp.
-
 S-adenosyl-L-homocysteine + 7-O-methylkaempferol
-
 ?
S-adenosyl-L-methionine + quercetin Populus sp.
-
 S-adenosyl-L-homocysteine + 7-O-methylquercetin
-
 ?

Organism

Organism UniProt Comment Textmining
Populus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged wild-type POMT7 and mutant POMT7-M1 from Escherichia coli by glutathione affinity chromatography Populus sp.
recombinent POMT7 and mutants Populus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-adenosyl-L-methionine + kaempferol activity only of enzyme mutant POMT7-M1, D257G, not of wild-type POMT7 Populus sp. S-adenosyl-L-homocysteine + 3,7-O-dimethylkaempferol
-
 ?
2 S-adenosyl-L-methionine + quercetin activity only of enzyme mutant POMT7-M1, D257G, not of wild-type POMT7, molecular docking of quercetin into the modeled structure of Asp257Gly Populus sp. S-adenosyl-L-homocysteine + 3,7-O-dimethylquercetin
-
 ?
S-adenosyl-L-methionine + 3,3',4',5,7-pentahydroxyflavone i.e. quercetin Populus sp. S-adenosyl-L-homocysteine + 3,3',4',5-tetrahydroxy-7-methoxyflavone
-
 ?
S-adenosyl-L-methionine + 3,4',5,7-tetrahydroxyflavanone i.e. kaempferol Populus sp. S-adenosyl-L-homocysteine + 3,4',5-trihydroxy-7-methoxyflavanone
-
 ?
S-adenosyl-L-methionine + kaempferol
-
 Populus sp. S-adenosyl-L-homocysteine + 7-O-methylkaempferol
-
 ?
S-adenosyl-L-methionine + quercetin
-
 Populus sp. S-adenosyl-L-homocysteine + 7-O-methylquercetin
-
 ?

Synonyms

Synonyms Comment Organism
flavonoid O-methyltransferase
-
 Populus sp.
POMT7
-
 Populus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Populus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.064
-
 3,4',5,7-tetrahydroxyflavanone i.e. kaempferol, pH 7.5, 37°C, wild-type enzyme Populus sp.
0.064
-
 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4H-1-benzopyran-4-one i.e. quercetin, pH 7.5, 37°C, mutant enzyme N257G Populus sp.
0.064
-
 kaempferol pH 7.5, 37°C, recombinant mutant POMT7-M1 Populus sp.
0.064
-
 quercetin pH 7.5, 37°C, recombinant wild-type POMT7 Populus sp.
0.07
-
 3,4',5,7-tetrahydroxyflavanone i.e. kaempferol, pH 7.5, 37°C, mutant enzyme N257G Populus sp.
0.07
-
 quercetin pH 7.5, 37°C, recombinant mutant POMT7-M1 Populus sp.
0.071
-
 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4H-1-benzopyran-4-one i.e. quercetin, pH 7.5, 37°C, wild-type enzyme Populus sp.
0.071
-
 kaempferol pH 7.5, 37°C, recombinant wild-type POMT7 Populus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Populus sp.

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Populus sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.7
-
 3,4',5,7-tetrahydroxyflavanone i.e. kaempferol, pH 7.5, 37°C, mutant enzyme N257G Populus sp.
1.7
-
 quercetin pH 7.5, 37°C, recombinant mutant POMT7-M1 Populus sp.
1.9
-
 3,4',5,7-tetrahydroxyflavanone i.e. kaempferol, pH 7.5, 37°C, wild-type enzyme Populus sp.
1.9
-
 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4H-1-benzopyran-4-one i.e. quercetin, pH 7.5, 37°C, mutant enzyme N257G Populus sp.
1.9
-
 kaempferol pH 7.5, 37°C, recombinant mutant POMT7-M1 Populus sp.
1.9
-
 quercetin pH 7.5, 37°C, recombinant wild-type POMT7 Populus sp.
2.4
-
 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4H-1-benzopyran-4-one i.e. quercetin, pH 7.5, 37°C, wild-type enzyme Populus sp.
2.4
-
 kaempferol pH 7.5, 37°C, recombinant wild-type POMT7 Populus sp.