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Literature summary for 2.1.1.95 extracted from

  • Koch, M.; Lemke, R.; Heise, K.P.; Mock, H.P.
    Characterization of gamma-tocopherol methyltransferases from Capsicum annuum L. and Arabidopsis thaliana (2003), Eur. J. Biochem., 270, 84-92.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
in Escherichia coli Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
alpha-tocopherol noncompetitive Arabidopsis thaliana
S-adenosyl-L-homocysteine noncompetitive Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 S-adenosyl-L-methionine
-
 Capsicum annuum
0.003
-
 gamma-tocopherol
-
 Capsicum annuum
0.003
-
 delta-tocopherol
-
 Arabidopsis thaliana
0.003
-
 delta-tocopherol
-
 Capsicum annuum
0.005
-
 S-adenosyl-L-methionine
-
 Arabidopsis thaliana
0.005
-
 gamma-tocopherol
-
 Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Capsicum annuum 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 SDS-PAGE Capsicum annuum
600000
-
 gel filtration Capsicum annuum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + gamma-tocopherol Arabidopsis thaliana biosynthesis of alpha-tocopherol S-adenosyl-L-homocysteine + alpha-tocopherol
-
 ?
S-adenosyl-L-methionine + gamma-tocopherol Capsicum annuum biosynthesis of alpha-tocopherol S-adenosyl-L-homocysteine + alpha-tocopherol
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Capsicum annuum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Capsicum annuum
recombinant enzyme from E. coli using His-tag Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Capsicum annuum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information beta-tocopherol not accepted as substrate Arabidopsis thaliana ?
-
 ?
additional information beta-tocopherol not accepted as substrate Capsicum annuum ?
-
 ?
S-adenosyl-L-methionine + delta-tocopherol
-
 Arabidopsis thaliana S-adenosyl-L-homocysteine + beta-tocopherol
-
 ?
S-adenosyl-L-methionine + delta-tocopherol
-
 Capsicum annuum S-adenosyl-L-homocysteine + beta-tocopherol
-
 ?
S-adenosyl-L-methionine + gamma-tocopherol
-
 Capsicum annuum S-adenosyl-L-homocysteine + alpha-tocopherol
-
 ?
S-adenosyl-L-methionine + gamma-tocopherol iso-ordered bi-bi type reaction Arabidopsis thaliana S-adenosyl-L-homocysteine + alpha-tocopherol
-
 ?
S-adenosyl-L-methionine + gamma-tocopherol biosynthesis of alpha-tocopherol Arabidopsis thaliana S-adenosyl-L-homocysteine + alpha-tocopherol
-
 ?
S-adenosyl-L-methionine + gamma-tocopherol biosynthesis of alpha-tocopherol Capsicum annuum S-adenosyl-L-homocysteine + alpha-tocopherol
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
34
-
-
 Arabidopsis thaliana
34
-
-
 Capsicum annuum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Capsicum annuum
8.5
-
-
 Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
5.5 10
-
 Arabidopsis thaliana
5.5 10
-
 Capsicum annuum