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Literature summary for 2.1.1.95 extracted from
- Isolation and properties of gamma-tocopherol methyltransferase in Euglena gracilis (1992), Biochim. Biophys. Acta, 1128, 220-226.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | slight stimulation | Euglena gracilis |  |
General Stability
| General Stability | Organism |
|---|---|
| very labile in absence of MnCl2, complete inactivation within 36 h at 4°C, 50% loss of activity after 7 h at 4°C, stabilized by addition of 1 mM MnCl2, only 15% loss of activity after 90 h at 4°C | Euglena gracilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Euglena gracilis | |
| Fe2+ | - |
Euglena gracilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
S-adenosyl-L-methionine | - |
Euglena gracilis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Euglena gracilis | 9507 | - |
| membrane | loosely bound to outer chloroplast membrane | Euglena gracilis | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no effect with Mg2+, Ca2+, K+ and Na+ | Euglena gracilis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 150000 | - |
gel filtration | Euglena gracilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + gamma-tocopherol | Euglena gracilis | biosynthesis of alpha-tocopherol | S-adenosyl-L-homocysteine + alpha-tocopherol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Euglena gracilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Euglena gracilis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.004 | - |
purified enzyme | Euglena gracilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + beta-tocopherol | 70% as effective as gamma-tocopherol | Euglena gracilis | ? | - |
? | |
| S-adenosyl-L-methionine + delta-tocopherol | 35% as effective as gamma-tocopherol | Euglena gracilis | S-adenosyl-L-homocysteine + beta-tocopherol | - |
? | |
| S-adenosyl-L-methionine + gamma-tocopherol | - |
Euglena gracilis | S-adenosyl-L-homocysteine + alpha-tocopherol | - |
? | |
| S-adenosyl-L-methionine + gamma-tocopherol | biosynthesis of alpha-tocopherol | Euglena gracilis | S-adenosyl-L-homocysteine + alpha-tocopherol | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Euglena gracilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Euglena gracilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Euglena gracilis |
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