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Literature summary for 2.1.1.9 extracted from

  • Schmidberger, J.; James, A.; Edwards, R.; Naismith, J.; OHagan, D.
    Halomethane biosynthesis: Structure of a SAM-dependent halide methyltransferase from arabidopsis thaliana (2010), Angew. Chem. Int. Ed. Engl., 49, 3646-3648.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.9

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged protein expressed in Escherichia coli Rosetta II (DE3) Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment) Organism
co-crystallized with S-adenosyl-L-homocysteine Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
V23C not conserved active site residue, slightly improved activity, lowered stability Arabidopsis thaliana
Y172F reduced efficiency with Cl- Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.099
-
 NCS- wild type protein, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
0.103
-
 NCS- V23C mutant protein, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
0.141
-
 NCS- Y172F mutant protein, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q0WP12
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography, His-tag remove Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0434
-
 substrate NCS-, V23C mutant protein, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
0.0436
-
 substrate NCS-, wild type protein, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
0.046
-
 substrate NCS-, Y172F mutant protein, pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
thiocyanate + S-adenosyl-L-methionine
-
 Arabidopsis thaliana methyl thiocyanate + S-adenosyl-L-homocysteine
-
 ?

Synonyms

Synonyms Comment Organism
SAM-dependent halide methyltransferase
-
 Arabidopsis thaliana