Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | overview: inhibition by analogs of S-adenosyl-L-homocysteine and analogs of S-adenosyl-L-methionine | Rattus norvegicus | |
N-ethylmaleimide | more than 50% inhibition at 0.2 mM | Rattus norvegicus | |
p-chloromercuribenzoate | more than 50% inhibition at 0.1 mM | Rattus norvegicus | |
S-3-deazaadenosyl-L-homocysteine | - |
Rattus norvegicus | |
S-adenosyl-L-homocysteine | - |
Rattus norvegicus | |
S-Tubercidinyl-L-homocysteine | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
S-Aristeromycinyl-L-methionine | - |
Rattus norvegicus | |
0.106 | - |
S-adenosyl-L-methionine | - |
Rattus norvegicus | |
0.12 | - |
S-3'-Deoxyadenosyl-L-methionine | - |
Rattus norvegicus | |
0.154 | - |
S-Tubercidinyl-L-methionine | - |
Rattus norvegicus | |
0.224 | - |
S-3-Deazaadenosyl-L-methionine | - |
Rattus norvegicus | |
0.719 | - |
S-8-Azaadenosyl-L-methionine | - |
Rattus norvegicus | |
1.017 | - |
S-N6-Methyladenosyl-L-methionine | - |
Rattus norvegicus | |
2.37 | - |
2-mercaptoethanol | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Rattus norvegicus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no stimulation by: Na+, K+, Mn2+ | Rattus norvegicus | |
additional information | no stimulation by: Mg2+ | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Storage Stability | Organism |
---|---|
4°C, 140 mM NaCl, 10 mM Tris-chloride, pH 7.4, half-life: 4 days, best conditions of storage | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-mercaptoethanol + S-adenosyl-L-methionine | - |
Rattus norvegicus | S-methyl-2-mercaptoethanol + S-adenosyl-L-homocysteine | - |
? | |
mercaptoacetic acid + S-adenosyl-L-methionine | i.e. thioglycolic acid | Rattus norvegicus | S-methylmercaptoacetic acid + S-adenosyl-L-homocysteine | - |
? | |
additional information | not: glutathione | Rattus norvegicus | ? | - |
? | |
additional information | not: cysteine | Rattus norvegicus | ? | - |
? | |
S-3'-deoxyadenosyl-L-methionine + 2-mercaptoethanol | - |
Rattus norvegicus | S-3'-deoxyadenosyl-L-homocysteine + S-methyl-2-mercaptoethanol | - |
? | |
S-3-deazaadenosyl-L-methionine + 2-mercaptoethanol | - |
Rattus norvegicus | S-3-deazaadenosyl-L-homocysteine + S-methyl-2-mercaptoethanol | - |
? | |
S-8-azaadenosyl-L-methionine + 2-mercaptoethanol | - |
Rattus norvegicus | S-8-azaadenosyl-L-homocysteine + S-methyl-2-mercaptoethanol | - |
? | |
S-adenosyl-L-methionine + a thiol | high specificity for S-adenosyl-L-methionine | Rattus norvegicus | S-adenosyl-L-homocysteine + a thioether | - |
? | |
S-aristeromycinyl-L-methionine + 2-mercaptoethanol | - |
Rattus norvegicus | S-aristeromycinyl-L-homocysteine + S-methyl-2-mercaptoethanol | - |
? | |
S-N6-methyladenosyl-L-methionine + 2-mercaptoethanol | - |
Rattus norvegicus | S-N6-methyladenosyl-L-homocysteine + S-methyl-2-mercaptoethanol | - |
? | |
S-tubercidinyl-L-methionine + 2-mercaptoethanol | - |
Rattus norvegicus | S-tubercidinyl-L-homocysteine + S-methyl-2-mercaptoethanol | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 9 | S-methylation of 2-mercaptoethanol | Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
30°C, absence of nucleotide substrate, half-life: less than 1 min | Rattus norvegicus |