Literature summary for 2.1.1.77 extracted from

Fang, P.; Li, X.; Wang, J.; Xing, L.; Gao, Y.; Niu, L.; Teng, M.
Crystal structure of the protein L-isoaspartyl methyltransferase from Escherichia coli (2010), Cell Biochem. Biophys., 58, 163-167.

Search for more literature for 2.1.1.77

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli strain B834 (DE3)	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with S-adenosyl-L-homocysteine, hanging drop vapor diffusion method, using 0.1 M HEPES-Na [pH 7.5], 0.8 M monosodium dihydrogen phosphate, and 0.8 M monopotassium dihydrogen phosphate, at 15°C	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
S-adenosyl-L-homocysteine	-	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
21900	-	1 * 21900, calculated from amino acid sequence	Escherichia coli
23300	-	gel filtration	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + protein D-isoaspartate	Escherichia coli	-	S-adenosyl-L-homocysteine + protein D-isoaspartate alpha-methyl ester	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0A7A5	-	-

Purification (Commentary)

Purification (Comment)	Organism
chelating Sepharose column chromatography	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + protein D-isoaspartate	-	Escherichia coli	S-adenosyl-L-homocysteine + protein D-isoaspartate alpha-methyl ester	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 21900, calculated from amino acid sequence	Escherichia coli

Synonyms

Synonyms	Comment	Organism
PIMT	-	Escherichia coli
protein L-isoaspartyl methyltransferase	-	Escherichia coli

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Release 2023.1 (January 2023)