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Literature summary for 2.1.1.76 extracted from

  • De Luca, V.; Ibrahim, R.K.
    Enzymatic synthesis of polymethylated flavonols in Chrysosplenium americanum. I. Partial purification and some properties of S-adenosyl-L-methionine:flavonol 3-, 6-, 7-, and 4-O-methyltransferases (1985), Arch. Biochem. Biophys., 238, 596-605.
    View publication on PubMed
Search for more literature for 2.1.1.76

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol relative activity 105% Chrysosplenium americanum
iodoacetamide relative activity 105% Chrysosplenium americanum

Inhibitors

Inhibitors Comment Organism Structure
N-ethylmaleimide
-
 Chrysosplenium americanum
p-chloromercuribenzoate
-
 Chrysosplenium americanum

Metals/Ions

Metals/Ions Comment Organism Structure
K+ relative activity 115% Chrysosplenium americanum
Mn2+ relative activity 110% Chrysosplenium americanum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 gel filtration Chrysosplenium americanum
65000
-
 gel filtration Chrysosplenium americanum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone Citrofortunella microcarpa
-
 S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone
-
 ?
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone Chrysosplenium americanum
-
 S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone 3-O-methylquercetin ?

Organism

Organism UniProt Comment Textmining
Chrysosplenium americanum
-
-
-
Citrofortunella microcarpa
-
 calamondin orange, synonym Citrofortunella mitis
-

Purification (Commentary)

Purification (Comment) Organism
partially Chrysosplenium americanum
partially Citrofortunella microcarpa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 53 pkat/mg Chrysosplenium americanum

Storage Stability

Storage Stability Organism
-15°C, purified enzyme, 0.2 M potassium phosphate, pH 8.0, 20% glycerol, 5 mM dithiothreitol, stable for several months Chrysosplenium americanum
4°C, partially purified enzyme loses 80% of its activity over a period of 4-6 weeks Chrysosplenium americanum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone
-
 Chrysosplenium americanum S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone
-
 ?
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone
-
 Citrofortunella microcarpa S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone
-
 ?
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone
-
 Chrysosplenium americanum S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone 3-O-methylquercetin ?
S-adenosyl-L-methionine + 7-methylquercetin i.e. rhamnetin, 30% of the activity with quercetin Chrysosplenium americanum ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8.2
-
 Chrysosplenium americanum

pH Range

pH Minimum pH Maximum Comment Organism
7.2 9.3 about 50% of activity maximum at pH 7.2 and 9.3 Chrysosplenium americanum