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Literature summary for 2.1.1.74 extracted from
- Expression and purification of untagged and histidine-tagged folate-dependent tRNA:m5U54 methyltransferase from Bacillus subtilis (2010), Protein Expr. Purif., 73, 83-89.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| untagged N-terminus and C-terminus (His)6-tagged TrmFO from Bacillus subtilis is expressed in Escherichia coli. The tag does not significantly alter the expression level, flavin content, activity and secondary structure of the protein | Bacillus subtilis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| R312G | mutant isolated after cloning is found to be defective in tRNA methylation, with an activity corresponding only to 40% that of the wild-type enzyme | Bacillus subtilis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33600 | - |
untagged protein | Bacillus subtilis |
| 47900 | - |
His-tagged fusion protein | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified to homogeneity by metal affinity or ion exchange and heparin affinity, respectively, followed by gel filtration | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + tRNA UpsiC + NADH | - |
Bacillus subtilis | tetrahydrofolate + tRNA TpsiC + NAD+ | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TRMFO | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Bacillus subtilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | is used in the assay as electron donor | Bacillus subtilis | |
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