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Literature summary for 2.1.1.74 extracted from

  • Delk, A.S.; Nagle, D.P.; Rabinowitz, J.C.
    The methylenetetrahydrofolate-mediated biosynthesis of ribothymidine in the transfer-RNA of Streptococcus faecalis: incorporation of hydrogen from solvent into the methyl moiety (1979), Biochem. Biophys. Res. Commun., 86, 244-251.
    View publication on PubMed
Search for more literature for 2.1.1.74

General Stability

General Stability Organism
enzyme requires FAD+ for stability Enterococcus faecalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + tRNA UpsiC + FADH2 Bacillus subtilis
-
 tetrahydrofolate + tRNA TpsiC + FAD
-
 ?
5,10-methylenetetrahydrofolate + tRNA UpsiC + FADH2 Enterococcus faecalis
-
 tetrahydrofolate + tRNA TpsiC + FAD
-
 ?
5,10-methylenetetrahydrofolate + tRNA UpsiC + FADH2 Bacillus cereus
-
 tetrahydrofolate + tRNA TpsiC + FAD
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-
Bacillus subtilis
-
-
-
Enterococcus faecalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Enterococcus faecalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.1
-
-
 Enterococcus faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + tRNA UpsiC + FADH2
-
 Bacillus subtilis tetrahydrofolate + tRNA TpsiC + FAD
-
 ?
5,10-methylenetetrahydrofolate + tRNA UpsiC + FADH2
-
 Enterococcus faecalis tetrahydrofolate + tRNA TpsiC + FAD
-
 ?
5,10-methylenetetrahydrofolate + tRNA UpsiC + FADH2
-
 Bacillus cereus tetrahydrofolate + tRNA TpsiC + FAD
-
 ?

Cofactor

Cofactor Comment Organism Structure
FADH2
-
 Bacillus subtilis
FADH2
-
 Enterococcus faecalis