KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | detailed kinetics for diverse substrate duplexes and methylation sites | Escherichia coli | |
additional information | - |
additional information | detailed kinetics for diverse substrate duplexes and methylation sites | Tequatrovirus T4 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + DNA adenine | Escherichia coli | - |
S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? | |
S-adenosyl-L-methionine + DNA adenine | Tequatrovirus T4 | - |
S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Tequatrovirus T4 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + DNA adenine | - |
Escherichia coli | S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? | |
S-adenosyl-L-methionine + DNA adenine | - |
Tequatrovirus T4 | S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? | |
S-adenosyl-L-methionine + DNA adenine | specific for methylation site sequence GATC and structure, an intact GA sequence is essential for activity, altered structural symmetry of the DNA substrate decreases kcat sharply, the best contact between enzyme and DNA is a palindromic interaction site covering the 5'-symmetric residues, which is located in the major groove, and another one in the 3'-half covering the 3'-symmetric residues is located in the minor groove, overview | Escherichia coli | S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? | |
S-adenosyl-L-methionine + DNA adenine | specific for methylation site sequence GATC and structure, an intact GAT sequence is essential for activity, altered structural symmetry of the DNA substrate decreases kcat sharply, the best contact between enzyme and DNA is a palindromic interaction site covering the 5'-symmetric residues, which is located in the major groove, and another one in the 3'-half covering the 3'-symmetric residues is located in the minor groove, overview | Tequatrovirus T4 | S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNA [amino]-methyltransferase | - |
Escherichia coli |
DNA [amino]-methyltransferase | - |
Tequatrovirus T4 |
DNA-[N6-adenine] MTase | - |
Escherichia coli |
DNA-[N6-adenine] MTase | - |
Tequatrovirus T4 |
EcoDam | - |
Escherichia coli |
EcoDam DNA-[N6-adenine] MTase | - |
Escherichia coli |
T4Dam | - |
Tequatrovirus T4 |
T4Dam DNA-[N6-adenine] MTase | - |
Tequatrovirus T4 |
[N6-adenine] MTase | - |
Escherichia coli |
[N6-adenine] MTase | - |
Tequatrovirus T4 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
25 | - |
assay at | Tequatrovirus T4 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |
8 | - |
assay at | Tequatrovirus T4 |