Cloned (Comment) | Organism |
---|---|
the enzyme is subcloned into the plasmid expression vector pJW2 | Tequatrovirus T4 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
methylated DNA | competitive | Tequatrovirus T4 | |
additional information | inhibited at ionic strength greater than 0.2 M | Tequatrovirus T4 | |
NEM | - |
Tequatrovirus T4 | |
S-adenosyl-L-homocysteine | competitive | Tequatrovirus T4 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000000011 | - |
adenine in T4 gt- dam- DNA | nonglucosylated unmethylated T4 gt- dam- DNA | Tequatrovirus T4 | |
0.0001 | - |
S-adenosyl-L-methionine | - |
Tequatrovirus T4 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | no requirement for Mg2+ | Tequatrovirus T4 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30400 | - |
x * 30400, SDS-PAGE | Tequatrovirus T4 |
30690 | - |
gel filtration, glycerol gradient centrifugation | Tequatrovirus T4 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Tequatrovirus T4 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Tequatrovirus T4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + adenine in T4 gt- dam- DNA | - |
Tequatrovirus T4 | S-adenosyl-L-homocysteine + N6-methyladenine in T4 gt- dam- DNA | - |
? | |
S-adenosyl-L-methionine + DNA adenine | nonglucosylated unmethylated T4 gt- dam- DNA. The enzyme methylates the palindromic tetranucleotide, GATC, designated the canonical sequence. At high Mtase:DNA ratios, T4 Dam can methylate some noncanonical sequences belonging to GAY, where Y represents cytosine or thymine | Tequatrovirus T4 | S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30400, SDS-PAGE | Tequatrovirus T4 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Tequatrovirus T4 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000000046 | - |
methylated DNA | - |
Tequatrovirus T4 | |
0.0024 | - |
S-adenosyl-L-homocysteine | - |
Tequatrovirus T4 |