Crystallization (Comment) | Organism |
---|---|
apo-form and binary complex with S-adenosyl-methionine, to 2.1 and 2.8 A resolution, respectively, and molecular modeling of the ternary complex structure with 5-hydroxyconiferaldehyde | Sorghum bicolor |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-hydroxyconiferaldehyde | - |
Sorghum bicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00086 | - |
5-hydroxyconiferaldehyde | pH 6.5, 22°C | Sorghum bicolor | |
0.0011 | - |
5-hydroxyconiferaldehyde | pH 7.5, 22°C | Sorghum bicolor | |
0.012 | - |
3,4-dihydroxy-trans-cinnamate | pH 6.5, 22°C | Sorghum bicolor | |
0.017 | - |
S-adenosyl-L-methionine | pH 6.5, 22°C | Sorghum bicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sorghum bicolor | C5YH12 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | random bi-bi mechanism. The observed reactivity hierarchy among 4,5-dihydroxy-3-methoxycinnamyl (and 3,4-dihydroxycinnamyl) aldehyde, alcohol, and acid substrates arises from the ability of the aldehyde to stabilize the anionic intermediate that results from deprotonation of the 5-hydroxyl group by histidine-267. Sinapaldehyde is the preferential product, as demonstrated by its low Km for 5-hydroxyconiferaldehyde. Unlike its acid and alcohol substrates, the aldehydes exhibit product inhibition, due to nonproductive binding of the S-cis-form of the aldehydes inhibiting productive binding of the S-trans-form | Sorghum bicolor | ? | - |
? | |
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate | - |
Sorghum bicolor | S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate | - |
? | |
S-adenosyl-L-methionine + 5-hydroxyconiferaldehyde | - |
Sorghum bicolor | S-adenosyl-L-homocysteine + 5-methoxyconiferaldehyde | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Sb07g003860 | - |
Sorghum bicolor |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0027 | - |
5-hydroxyconiferaldehyde | pH 7.5, 22°C | Sorghum bicolor | |
0.01 | - |
5-hydroxyconiferaldehyde | pH 6.5, 22°C | Sorghum bicolor |