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Literature summary for 2.1.1.68 extracted from
- Evolution of novel O-methyltransferases from the Vanilla planifolia caffeic acid O-methyltransferase (2006), Plant Mol. Biol., 61, 537-552.
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | Vanilla planifolia OMT-2 is most similar to caffeic acid O-methyltransferases, reveals new instance of COMT-like enzyme with novel substrate preferences | Vanilla planifolia |
| additional information | Vanilla planifolia OMT-3 is most similar to caffeic acid O-methyltransferases, reveals new instance of COMT-like enzyme with novel substrate preferences | Vanilla planifolia |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression as N-terminal polyhistidine-tagged fusion in Escherichia coli BL21-CodonPlus (DE3)-RIPL competent cells based on the expression vector pET-15bexpression | Vanilla planifolia |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.114 | - |
myricetin | - |
Vanilla planifolia |  |
| 0.96 | - |
methyl gallate | - |
Vanilla planifolia | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38770 | - |
sequence analysis | Vanilla planifolia |
| 38920 | - |
sequence analysis | Vanilla planifolia |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vanilla planifolia | Q27I62 | - |
- |
| Vanilla planifolia | Q27I63 | - |
- |
| Vanilla pompona | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by nickel affinity chromatography | Vanilla planifolia |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | - |
Vanilla planifolia | - |
| additional information | no activity in stem, leaf and bean | Vanilla planifolia | - |
| root | - |
Vanilla pompona | - |
| root | most highly expressed | Vanilla planifolia | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, Tris-HCl buffer, pH 7.5 | Vanilla planifolia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| delphinidin | - |
Vanilla planifolia | ? | - |
? | |
| ethyl gallate | - |
Vanilla planifolia | ? | - |
? | |
| methyl gallate | - |
Vanilla planifolia | ? | - |
? | |
| additional information | does not have significant activity with the typical COMT substrates, caffeoyl aldehyde, 5-OH-coniferaldehyde, 5-OH-ferulic acid or caffeic acid or with the proposed vanillin precursor 3,4-dihydroxybenzaldehyde, thus is no component of the vanillin biosynthetic pathway, also no activity with kaempferol or quercetin | Vanilla planifolia | ? | - |
? | |
| myricetin | - |
Vanilla planifolia | ? | - |
? | |
| myricitrin | - |
Vanilla planifolia | ? | - |
? | |
| n-propyl gallate | best substrate | Vanilla planifolia | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| caffeic acid O-methyltransferase | - |
Vanilla planifolia |
| caffeic acid O-methyltransferase | - |
Vanilla pompona |
| COMT | - |
Vanilla planifolia |
| COMT | - |
Vanilla pompona |
| Van OMT-2 | - |
Vanilla planifolia |
| Van OMT-2 | - |
Vanilla pompona |
| Van OMT-3 | - |
Vanilla planifolia |
| Van OMT-3 | - |
Vanilla pompona |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.068 | - |
myricetin | - |
Vanilla planifolia | |
| 0.23 | - |
methyl gallate | - |
Vanilla planifolia | |
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Release 2023.1 (January 2023)
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