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Literature summary for 2.1.1.68 extracted from

  • Lin, F.; Yamano, G.; Hasegawa, M.; Anzai, H.; Kawasaki, S.; Kodama, O.
    Cloning and functional analysis of caffeic acid 3-O-methyltransferase from rice (Oryza sativa) (2006), J. Pestic. Sci., 31, 47-53.
No PubMed abstract available
Search for more literature for 2.1.1.68

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Oryza sativa

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ relative activity 70% of control Oryza sativa
Ca2+ relative activity 58% of control Oryza sativa
Co2+ strongly inhibits, relative activity 7% of control Oryza sativa
Cu2+ relative activity 0% of control Oryza sativa
EDTA relative activity 77% of control Oryza sativa
Hg2+ relative activity 0% of control Oryza sativa
Mg2+ relative activity 64% of control Oryza sativa
Mn2+ relative activity 24% of control Oryza sativa
additional information no increase in the expression of OsCOMT1 mRNA detected in CuCl2-, jasmonic acid and UV treated leaves Oryza sativa
Zn2+ relative activity 9% of control Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.051
-
 S-adenosyl-L-methionine when caffeic acid is supplied at a constant concentration of 1 mM Oryza sativa
0.069
-
 caffeic acid when S-adenosyl-L-methionine is supplied at a constant concentration of 1 mM Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 purified enzyme, SDS-PAGE Oryza sativa
42300
-
 sequence analysis Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by His-Bind column chromatography Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
aerial part
-
 Oryza sativa
-
leaf
-
 Oryza sativa
-
root
-
 Oryza sativa
-

Storage Stability

Storage Stability Organism
-80°C, Tris-HCl buffer, 10% glycerol and 2-mercaptoethanol, pH 8.0 Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
eriodictyol best substrate, 131% relative activity Oryza sativa ?
-
 ?
luteolin 88% relative activity Oryza sativa ?
-
 ?
additional information no acitivity with p-coumaric acid flavanones, naringenin, homoeriodictyol, hesperetin flavonols, kaempferol, isorhametin flavones, apigenin and chrysoeriol Oryza sativa ?
-
 ?
myricetin 75% relative activity Oryza sativa ?
-
 ?
quercetin 71% relative activity Oryza sativa ?
-
 ?
S-adenosyl-L-methionine + caffeic acid 100% relative activity Oryza sativa ?
-
 ?

Synonyms

Synonyms Comment Organism
caffeic acid 3-O-methyltransferase
-
 Oryza sativa
OsCOMT1 previously reported as NOMT Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Oryza sativa

pH Range

pH Minimum pH Maximum Comment Organism
5 9
-
 Oryza sativa