Application | Comment | Organism |
---|---|---|
synthesis | engineered COMT enzymes can be useful for metabolic engineering of both lignin and benzaldehyde-derived flavors and fragances | Medicago sativa |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Medicago sativa |
Protein Variants | Comment | Organism |
---|---|---|
A162T | 2fold increase in ratio of Vmax to Km-value for caffeic acid compared to wild-type value, 2.9fold decrease in ratio of Vmax to Km-value for 5-hydroxy coniferaldehyde compared to wild-type enzyme, 1.8fold decrease in ratio of Vmax to Km-value for protocatechuic aldehyde compared to wild-type enzyme, 2.8fold decrease in ratio of Vmax to Km-value for 3,4-dihydroxy-5-methoxybenzaldehyde compared to wild-type enzyme, no activity against protocatechuic acid | Medicago sativa |
F172Y | 3fold decrease in ratio of Vmax to Km-value for 5-hydroxy coniferaldehyde compared to wild-type enzyme, 34fold decrease in ratio of Vmax to Km-value for protocatechuic aldehyde compared to wild-type enzyme, 2.3fold decrease in ratio of Vmax to Km-value for 3,4-dihydroxy-5-methoxybenzaldehyde compared to wild-type enzyme, no activity with caffeic acid and protocatechuic acid | Medicago sativa |
H183K | 19fold decrease in ratio of Vmax to Km-value for caffeic acid compared to wild-type value, 1.8fold decrease in ratio of Vmax to Km-value for 5-hydroxy coniferaldehyde compared to wild-type enzyme, no activity with protocatechuic aldehyde, 3,4-dihydroxy-5-methoxybenzaldehyde and protocatechuic acid | Medicago sativa |
L136Y | 1.5 fold increase in ratio of Vmax to Km-value for caffeic acid compared to wild-type value, 4.8fold decrease in ratio of Vmax to Km-value for 5-hydroxy coniferaldehyde compared to wild-type enzyme, 1.7fold decrease in ratio of Vmax to Km-value for protocatechuic aldehyde compared to wild-type enzyme, 3.9fold decrease in ratio of Vmax to Km-value for 3,4-dihydroxy-5-methoxybenzaldehyde compared to wild-type enzyme, 176fold decrease in ratio of Vmax to Km-value for protocatechuic acid compared to wild-type enzyme | Medicago sativa |
M130L | 3.1fold decrease in ratio of Vmax to Km-value for 5-hydroxy coniferaldehyde compared to wild-type enzyme, 51fold decrease in ratio of Vmax to Km-value for protocatechuic aldehyde compared to wild-type enzyme, 5.8fold decrease in ratio of Vmax to Km-value for 3,4-dihydroxy-5-methoxybenzaldehyde compared to wild-type enzyme,no activity with caffeic acid and protocatechuic acid | Medicago sativa |
N131D | 9.5fold decrease in ratio of Vmax to Km-value for caffeic acid compared to wild-type value, 2.4fold decrease in ratio of Vmax to Km-value for 5-hydroxy coniferaldehyde compared to wild-type enzyme, no activity with protocatechuic aldehyde, 3,4-dihydroxy-5-methoxybenzaldehyde and protocatechuic acid | Medicago sativa |
N131E | 475fold decrease in ratio of Vmax to Km-value for caffeic acid compared to wild-type value, 1.7fold increase in ratio of Vmax to Km-value for 5-hydroxy coniferaldehyde compared to wild-type enzyme, no activity with protocatechuic aldehyde, 3,4-dihydroxy-5-methoxybenzaldehyde and protocatechuic acid | Medicago sativa |
N131K | 3.2 fold increase in ratio of Vmax to Km-value for caffeic acid compared to wild-type value, 2.9fold decrease in ratio of Vmax to Km-value for 5-hydroxy coniferaldehyde compared to wild-type enzyme, 1.7fold decrease in ratio of Vmax to Km-value for protocatechuic aldehyde compared to wild-type enzyme, 2.8fold decrease in ratio of Vmax to Km-value for 3,4-dihydroxy-5-methoxybenzaldehyde compared to wild-type enzyme, 3.3fold decrease in ratio of Vmax to Km-value for protocatechuic acid compared to wild-type enzyme | Medicago sativa |
N131L | 23.8fold decrease in ratio of Vmax to Km-value for caffeic acid compared to wild-type value, 2.5fold decrease in ratio of Vmax to Km-value for 5-hydroxy coniferaldehyde compared to wild-type enzyme, 5.7fold decrease in ratio of Vmax to Km-value for protocatechuic aldehyde compared to wild-type enzyme, 1.5fold decrease in ratio of Vmax to Km-value for 3,4-dihydroxy-5-methoxybenzaldehyde compared to wild-type enzyme, no activity with protocatechuic acid | Medicago sativa |
N324H/M130L | 2.4fold decrease in ratio of Vmax to Km-value for caffeic acid compared to wild-type value, 4.2fold decrease in ratio of Vmax to Km-value for 5-hydroxy coniferaldehyde compared to wild-type enzyme, 2.8fold decrease in ratio of Vmax to Km-value for protocatechuic aldehyde compared to wild-type enzyme, 3.1fold decrease in ratio of Vmax to Km-value for 3,4-dihydroxy-5-methoxybenzaldehyde compared to wild-type enzyme, 6.7fold decrease in ratio of Vmax to Km-value for protocatechuic acid compared to wild-type enzyme | Medicago sativa |
N324Y | 31.7fold decrease in ratio of Vmax to Km-value for caffeic acid compared to wild-type value, 2.4fold decrease in ratio of Vmax to Km-value for 5-hydroxy coniferaldehyde compared to wild-type enzyme, 10.2fold decrease in ratio of Vmax to Km-value for protocatechuic aldehyde compared to wild-type enzyme, 1.7fold decrease in ratio of Vmax to Km-value for 3,4-dihydroxy-5-methoxybenzaldehyde compared to wild-type enzyme, no activity with protocatechuic acid | Medicago sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
5-hydroxyconiferaldehyde | mutant enzyme N131E | Medicago sativa | |
0.005 | - |
Protocatechuic aldehyde | wild-type enzyme | Medicago sativa | |
0.005 | - |
5-hydroxyconiferaldehyde | wild-type enzyme | Medicago sativa | |
0.005 | - |
3,4-dihydroxy-5-methoxy-benzaldehyde | mutant enzyme N324Y | Medicago sativa | |
0.006 | - |
Protocatechuic aldehyde | mutant enzyme N324H/M130L | Medicago sativa | |
0.006 | - |
Protocatechuic aldehyde | mutant enzyme N324Y | Medicago sativa | |
0.008 | - |
5-hydroxyconiferaldehyde | mutant enzyme H183K | Medicago sativa | |
0.009 | - |
caffeic acid | mutant enzyme N131K | Medicago sativa | |
0.01 | - |
Protocatechuic aldehyde | mutant enzyme A162T | Medicago sativa | |
0.01 | - |
5-hydroxyconiferaldehyde | mutant enzyme F172Y | Medicago sativa | |
0.01 | - |
caffeic acid | mutant enzyme L136Y | Medicago sativa | |
0.01 | - |
Protocatechuic aldehyde | mutant enzyme N131L | Medicago sativa | |
0.011 | - |
5-hydroxyconiferaldehyde | mutant enzyme A162T | Medicago sativa | |
0.012 | - |
caffeic acid | mutant enzyme A162T | Medicago sativa | |
0.012 | - |
3,4-dihydroxy-5-methoxy-benzaldehyde | mutant enzyme M130L | Medicago sativa | |
0.012 | - |
5-hydroxyconiferaldehyde | mutant enzyme N131K | Medicago sativa | |
0.013 | - |
5-hydroxyconiferaldehyde | mutant enzyme L136Y | Medicago sativa | |
0.013 | - |
5-hydroxyconiferaldehyde | mutant enzyme N131L | Medicago sativa | |
0.013 | - |
3,4-dihydroxy-5-methoxy-benzaldehyde | mutant enzyme N131L | Medicago sativa | |
0.015 | - |
Protocatechuic aldehyde | mutant enzyme L136Y | Medicago sativa | |
0.016 | - |
3,4-dihydroxy-5-methoxy-benzaldehyde | wild-type enzyme | Medicago sativa | |
0.017 | - |
5-hydroxyconiferaldehyde | mutant enzyme N324Y | Medicago sativa | |
0.019 | - |
Protocatechuic aldehyde | mutant enzyme M130L | Medicago sativa | |
0.027 | - |
3,4-dihydroxy-5-methoxy-benzaldehyde | mutant enzyme F172Y | Medicago sativa | |
0.028 | - |
5-hydroxyconiferaldehyde | mutant enzyme M130L | Medicago sativa | |
0.031 | - |
3,4-dihydroxy-5-methoxy-benzaldehyde | mutant enzyme L136Y | Medicago sativa | |
0.032 | - |
caffeic acid | mutant enzyme N324H/M130L | Medicago sativa | |
0.039 | - |
3,4-dihydroxy-5-methoxy-benzaldehyde | mutant enzyme A162T | Medicago sativa | |
0.041 | - |
5-hydroxyconiferaldehyde | mutant enzyme N324H/M130L | Medicago sativa | |
0.043 | - |
caffeic acid | wild-type enzyme | Medicago sativa | |
0.052 | - |
protocatechuic acid | mutant enzyme L136Y | Medicago sativa | |
0.055 | - |
Protocatechuic aldehyde | mutant enzyme N131K | Medicago sativa | |
0.057 | - |
3,4-dihydroxy-5-methoxy-benzaldehyde | mutant enzyme N131K | Medicago sativa | |
0.087 | - |
3,4-dihydroxy-5-methoxy-benzaldehyde | mutant enzyme N324H/M130L | Medicago sativa | |
0.12 | - |
5-hydroxyconiferaldehyde | mutant enzyme N131D | Medicago sativa | |
0.121 | - |
caffeic acid | mutant enzyme N131D | Medicago sativa | |
0.133 | - |
caffeic acid | mutant enzyme N324Y | Medicago sativa | |
0.14 | - |
caffeic acid | mutant enzyme H183K | Medicago sativa | |
0.153 | - |
Protocatechuic aldehyde | mutant enzyme F172Y | Medicago sativa | |
0.214 | - |
caffeic acid | mutant enzyme N131E | Medicago sativa | |
0.335 | - |
caffeic acid | mutant enzyme N131L | Medicago sativa | |
0.352 | - |
protocatechuic acid | mutant enzyme N131K | Medicago sativa | |
0.357 | - |
protocatechuic acid | mutant enzyme N324H/M130L | Medicago sativa | |
0.515 | - |
protocatechuic acid | wild-type enzyme | Medicago sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Medicago sativa | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with 3-hydroxybenzaldehyde, 4-hydroxybenzaldehyde, vanillin and syringaldehyde | Medicago sativa | ? | - |
? | |
S-adenosyl-L-methionine + 3,4-dihydroxy-5-methoxy-benzaldehyde | - |
Medicago sativa | S-adenosyl-L-homocysteine + 4-hydroxy-3,5-dimethoxybenzaldehyde | - |
? | |
S-adenosyl-L-methionine + 5-hydroxyconiferaldehyde | - |
Medicago sativa | S-adenosyl-L-homocysteine + 5-methoxyconiferaldehyde | - |
? | |
S-adenosyl-L-methionine + caffeic acid | - |
Medicago sativa | S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxy-trans-cinnamic acid | - |
? | |
S-adenosyl-L-methionine + protocatechuic acid | - |
Medicago sativa | S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxybenzoic acid | - |
? | |
S-adenosyl-L-methionine + protocatechuic aldehyde | - |
Medicago sativa | S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxybenzaldehyde | - |
? |
Synonyms | Comment | Organism |
---|---|---|
caffeic acid/5-hydroxyferulic acid 3/5-O-methyltransferase | - |
Medicago sativa |
COMT | - |
Medicago sativa |