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Literature summary for 2.1.1.67 extracted from

  • Woodson, L.C.; Weinshilboum, R.M.
    Human kidney thiopurine methyltransferase. Purification and biochemical properties (1983), Biochem. Pharmacol., 32, 819-826.
    View publication on PubMed
Search for more literature for 2.1.1.67

Activating Compound

Activating Compound Comment Organism Structure
SKF-525A
-
 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
6-methylmercaptopurine
-
 Homo sapiens
Ethacrynic acid
-
 Homo sapiens
N-ethylmaleimide
-
 Homo sapiens
S-adenosyl-L-homocysteine
-
 Homo sapiens
tropolone
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0027
-
 S-adenosyl-L-methionine
-
 Homo sapiens
0.3
-
 6-Mercaptopurine
-
 Homo sapiens
0.55
-
 6-thioguanine
-
 Homo sapiens
2
-
 2-Thiouracil
-
 Homo sapiens
168
-
 2-mercaptoethanol
-
 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ baseline activity 102% Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + a thiopurine Homo sapiens S-methylation, enzyme of major catabolic pathway of thiopurines S-adenosyl-L-homocysteine + a thiopurine S-methylether
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 human
-

Purification (Commentary)

Purification (Comment) Organism
partially Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
blood
-
 Homo sapiens
-
erythrocyte
-
 Homo sapiens
-
kidney
-
 Homo sapiens
-
lymphocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.03
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information aliphatic thiol compounds are poor substrates or not methylated, dithiothreitol is no substrate Homo sapiens ?
-
 ?
S-adenosyl-L-methionine + 2-mercaptoethanol
-
 Homo sapiens S-adenosyl-L-homocysteine + 2-methylmercaptoethanol
-
 ?
S-adenosyl-L-methionine + 2-thiouracil
-
 Homo sapiens S-adenosyl-L-homocysteine + thiouracil-2-S-methylether
-
 ?
S-adenosyl-L-methionine + 6-mercaptopurine
-
 Homo sapiens S-adenosyl-L-homocysteine + 6-methylmercaptopurine
-
 ?
S-adenosyl-L-methionine + 6-thioguanine
-
 Homo sapiens S-adenosyl-L-homocysteine + 6-methylthioguanine
-
 ?
S-adenosyl-L-methionine + 6-thiopurine
-
 Homo sapiens S-adenosyl-L-homocysteine + 6-methylthiopurine
-
 ?
S-adenosyl-L-methionine + a thiopurine
-
 Homo sapiens S-adenosyl-L-homocysteine + a thiopurine S-methylether
-
 ?
S-adenosyl-L-methionine + a thiopurine S-methylation, enzyme of major catabolic pathway of thiopurines Homo sapiens S-adenosyl-L-homocysteine + a thiopurine S-methylether
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7
-
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00075
-
 S-adenosyl-L-homocysteine
-
 Homo sapiens
0.85
-
 tropolone
-
 Homo sapiens