Literature summary for 2.1.1.63 extracted from

Heinis, C.; Schmitt, S.; Kindermann, M.; Godin, G.; Johnsson, K.
Evolving the substrate specificity of O6-alkylguanine-DNA alkyltransferase through loop insertion for applications in molecular imaging (2006), ACS Chem. Biol., 1, 575-584.

Search for more literature for 2.1.1.63

Application

Application	Comment	Organism
analysis	strategy of loop insertion to alter enzyme specificity shall be general and applicable to other classes of proteins, the isolated AGT mutant can be applied in molecular imaging, where the mutant and parental AGTs are used to label two different AGT fusion proteins with different fluorophores in the same living cell or in vitro, allows establishment of fluorescence-based assays to detect protein-protein interactions and measure enzymatic activities	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
G160W	cells overexpressing W160AGT do not become labeled, even when incubated with O6-propargylguanine for extended periods of time	Homo sapiens
additional information	insertion of random amino acid loops into the protein backbone reveals mutants that react with the non-natural substrate O6-propargylguanine, libraries generated by conventional random or targeted saturation mutagenesis, by contrast, do not yield any mutants with activity towards this new substrate	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
O6-benzylguanine	-	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
mutants purified by either nickel or glutathione affinity chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	low reactivity of the non-natural substrate O6-propargylguanine with AGT, most likely arises from the poor binding of the substrate to the active site of AGT	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
AGT	-	Homo sapiens
O6-alkylguanine-DNA alkyltransferase	-	Homo sapiens

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Release 2023.1 (January 2023)