Cloned (Comment) | Organism |
---|---|
cDNA is cloned into a pET-11a plasmid and expressed in Escherichia coli | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA containing 6-O-methylguanine + [protein]-L-cysteine | the binding to DNA is the rate determining step in the repair process. Approximately eight base pairs of the DNA substrate are covered by the human enzyme. Binding affinity to methylated DNA is two times higher than that to unmodified DNA. The interaction with DNA induces a conformational change in the enzyme | Homo sapiens | DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine | - |
? |