Literature summary for 2.1.1.63 extracted from

Leclere, M.M.; Nishioka, M.; Yuasa, T.; Fujiwara, S.; Takagi, M.; Imanaka, T.
The O6-methylguanine-DNA methyltransferase from the hyperthermophilic archaeon Pyrococcus sp. KOD1: a thermostable repair enzyme (1998), Mol. Gen. Genet., 258, 69-77.

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Cloned(Commentary)

Cloned (Comment)	Organism
the gene is placed under the control of the lac promoter and overexpressed in the methyltransferase-deficient Escherichia coli strain KT233	Pyrococcus sp.

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus sp.	-	KOD1	-
Pyrococcus sp. KOD1	-	KOD1	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Pyrococcus sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DNA containing 6-O-methylguanine + [protein]-L-cysteine	-	Pyrococcus sp.	DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine	-	?
DNA containing 6-O-methylguanine + [protein]-L-cysteine	-	Pyrococcus sp. KOD1	DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine	-	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
75	-	60 min, 25% loss of activity	Pyrococcus sp.
90	-	stable for at least 30 min. 66% loss of activity after 60 min	Pyrococcus sp.

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Release 2023.1 (January 2023)