Cloned (Comment) | Organism |
---|---|
the gene is placed under the control of the lac promoter and overexpressed in the methyltransferase-deficient Escherichia coli strain KT233 | Pyrococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus sp. | - |
KOD1 | - |
Pyrococcus sp. KOD1 | - |
KOD1 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Pyrococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA containing 6-O-methylguanine + [protein]-L-cysteine | - |
Pyrococcus sp. | DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine | - |
? | |
DNA containing 6-O-methylguanine + [protein]-L-cysteine | - |
Pyrococcus sp. KOD1 | DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
60 min, 25% loss of activity | Pyrococcus sp. |
90 | - |
stable for at least 30 min. 66% loss of activity after 60 min | Pyrococcus sp. |