Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coliKT233 and in HeLa cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | three allelic variants: V1 with amino acid substitution Leu84Phe, variant V2 with amino acid substitution Trp65Cys and variant V3 with a silent mutation. Wild-type and V1 variant have similar enzymatic and physicochemical properties, while variant V2 is considered to be unstable and rare | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA containing 6-O-methylguanine + [protein]-L-cysteine | Homo sapiens | the enzyme is a critical defense against alkylation-induced mutagenesis and carcinogenesis | DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
three allelic variants: V1, V2, and V3 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fibroblast | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA containing 6-O-methylguanine + [protein]-L-cysteine | - |
Homo sapiens | DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine | - |
? | |
DNA containing 6-O-methylguanine + [protein]-L-cysteine | the enzyme is a critical defense against alkylation-induced mutagenesis and carcinogenesis | Homo sapiens | DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine | - |
? |