Literature summary for 2.1.1.63 extracted from

Terashima, I.; Kawate, H.; Sakumi, K.; Sekiguchi, M.; Kohda, K.
Substrate specificity of human O6-methylguanine-DNA methyltransferase for O6-benzylguanine derivatives in oligodeoxynucleotides (1997), Chem. Res. Toxicol., 10, 1234-1239.

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Inhibitors

Inhibitors	Comment	Organism	Structure
double-stranded oligonucleotides	6-O-methylguanine, 6-O-(4-fluorobenzyl)-guanine, 6-O-(3-fluorobenzyl)-guanine, 6-O-(2-fluorobenzyl)-guanine, 6-O-benzylguanine, 6-O-benzylhypoxanthine. IC50: 1.4-3.0 nM	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DNA containing 6-O-methylguanine + [protein]-L-cysteine	oligonucleotides containing an 6-O-(2-fluorobenzyl)guanine, 6-O-(3-fluorobenzyl)guanine, 6-O-benzylhypoxanthine or 6-O-methylguanine are all good substrates	Homo sapiens	DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine	-	?

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0000014	0.000003	6-O-methylguanine, 6-O-(4-fluorobenzyl)-guanine, 6-O-(3-fluorobenzyl)-guanine, 6-O-(2-fluorobenzyl)-guanine, 6-O-benzylguanine, 6-O-benzylhypoxanthine. IC50: 1.4-3.0 nM	Homo sapiens	double-stranded oligonucleotides

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Release 2023.1 (January 2023)