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Literature summary for 2.1.1.6 extracted from

  • Bai, H.W.; Shim, J.Y.; Yu, J.; Zhu, B.T.
    Biochemical and molecular modeling studies of the O-methylation of various endogenous and exogenous catechol substrates catalyzed by recombinant human soluble and membrane-bound catechol-O-methyltransferases (2007), Chem. Res. Toxicol., 20, 1409-1425.
    View publication on PubMed
Search for more literature for 2.1.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expression of the human soluble and membrane-bound COMTs (S-COMT and MBCOMT, respectively) in Escherichia coli. The two recombinant human COMTs are functionally active, with catalytic and kinetic properties nearly identical to that of crude or purified enzymes prepared from human tissues or cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0015
-
 quercetin S-COMT, 0.25 mM S-adenosyl-L-methionine Homo sapiens
0.0015
-
 fisetin S-COMT, 0.25 mM S-adenosyl-L-methionine Homo sapiens
0.0022
-
 catechin MB-COMT, 0.25 mM S-adenosyl-L-methionine Homo sapiens
0.0032
-
 2-hydroxyestradiol MB-COMT, 0.1 mM S-adenosyl-L-methionine Homo sapiens
0.0036
-
 2-hydroxyestradiol S-COMT, 0.1 mM S-adenosyl-L-methionine Homo sapiens
0.0043
-
 epicatechin MB-COMT, 0.25 mM S-adenosyl-L-methionine Homo sapiens
0.0045
-
 4-hydroxyestradiol S-COMT, 0.1 mM S-adenosyl-L-methionine Homo sapiens
0.0058
-
 S-adenosyl-L-methionine cosubstrate: 4-hydroxyestradiol, MB-COMT Homo sapiens
0.0064
-
 4-hydroxyestradiol MB-COMT, 0.1 mM S-adenosyl-L-methionine Homo sapiens
0.0089
-
 catechin S-COMT, 0.25 mM S-adenosyl-L-methionine Homo sapiens
0.0144
-
 S-adenosyl-L-methionine cosubstrate: 2-hydroxyestradiol, MB-COMT Homo sapiens
0.017
-
 dopamine MB-COMT, 0.1 mM S-adenosyl-L-methionine Homo sapiens
0.0257
-
 epicatechin S-COMT, 0.25 mM S-adenosyl-L-methionine Homo sapiens
0.0288
-
 epinephrine MB-COMT, 0.1 mM S-adenosyl-L-methionine Homo sapiens
0.0306
-
 norepinephrine MB-COMT, 0.1 mM S-adenosyl-L-methionine Homo sapiens
0.1136
-
 S-adenosyl-L-methionine cosubstrate: 2-hydroxyestradiol, S-COMT Homo sapiens
0.1453
-
 S-adenosyl-L-methionine cosubstrate: 4-hydroxyestradiol, S-COMT Homo sapiens
0.224
-
 norepinephrine S-COMT, 0.1 mM S-adenosyl-L-methionine Homo sapiens
0.2632
-
 dopamine S-COMT, 0.1 mM S-adenosyl-L-methionine Homo sapiens
0.3947
-
 epinephrine S-COMT, 0.1 mM S-adenosyl-L-methionine Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane membrane-bound enzyme form MB-COMT Homo sapiens 16020
-
soluble soluble enzyme form S-COMT Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 1 * 24000, S-COMT, SDS-PAGE Homo sapiens
30000
-
 x * 30000, MB-COMT, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 recombinant
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxyestradiol + S-adenosyl-L-methionine MB-COMT methylates both 2-hydroxyestradiol and 4-hydroxyestradiol at comparable rates Homo sapiens S-adenosyl-L-homocysteine + ?
-
 ?
2-hydroxyestradiol + S-adenosyl-L-methionine S-COMT methylates 2-hydroxyestradiol roughly twice as fast as it methylates 4-hydroxyestradiol Homo sapiens S-adenosyl-L-homocysteine + ?
-
 ?
4-hydroxyestradiol + S-adenosyl-L-methionine MB-COMT methylates both 2-hydroxyestradiol and 4-hydroxyestradiol at comparable rates Homo sapiens S-adenosyl-L-homocysteine + ?
-
 ?
4-hydroxyestradiol + S-adenosyl-L-methionine S-COMT methylates 2-hydroxyestradiol roughly twice as fast as it methylates 4-hydroxyestradiol Homo sapiens S-adenosyl-L-homocysteine + ?
-
 ?
dopamine + S-adenosyl-L-methionine membrane-bound enzyme form MB-COMT Homo sapiens S-adenosyl-L-homocysteine + ?
-
 ?
dopamine + S-adenosyl-L-methionine soluble enzyme form S-COMT Homo sapiens S-adenosyl-L-homocysteine + ?
-
 ?
epinephrine + S-adenosyl-L-methionine membrane-bound enzyme form MB-COMT Homo sapiens metanephrine + S-adenosyl-L-homocysteine
-
 ?
epinephrine + S-adenosyl-L-methionine soluble enzyme form S-COMT Homo sapiens metanephrine + S-adenosyl-L-homocysteine
-
 ?
S-adenosyl-L-methionine + catechin membrane-bound enzyme form MB-COMT Homo sapiens S-adenosyl-L-homocysteine + ?
-
 ?
S-adenosyl-L-methionine + catechin soluble enzyme form S-COMT Homo sapiens S-adenosyl-L-homocysteine + ?
-
 ?
S-adenosyl-L-methionine + epicatechin membrane-bound enzyme form MB-COMT Homo sapiens S-adenosyl-L-homocysteine + ?
-
 ?
S-adenosyl-L-methionine + epicatechin soluble enzyme form S-COMT Homo sapiens S-adenosyl-L-homocysteine + ?
-
 ?
S-adenosyl-L-methionine + fisetin membrane-bound enzyme form MB-COMT Homo sapiens S-adenosyl-L-homocysteine + ?
-
 ?
S-adenosyl-L-methionine + fisetin soluble enzyme form S-COMT Homo sapiens S-adenosyl-L-homocysteine + ?
-
 ?
S-adenosyl-L-methionine + norepinephrine membrane-bound enzyme form MB-COMT Homo sapiens S-adenosyl-L-homocysteine + ?
-
 ?
S-adenosyl-L-methionine + norepinephrine soluble enzyme form S-COMT Homo sapiens S-adenosyl-L-homocysteine + ?
-
 ?
S-adenosyl-L-methionine + quercetin membrane-bound enzyme form MB-COMT Homo sapiens S-adenosyl-L-homocysteine + ?
-
 ?
S-adenosyl-L-methionine + quercetin soluble enzyme form S-COMT Homo sapiens S-adenosyl-L-homocysteine + ?
-
 ?

Subunits

Subunits Comment Organism
? 1 * 24000, S-COMT, SDS-PAGE Homo sapiens
? x * 30000, MB-COMT, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
MB-COMT membrane-bound enzyme form Homo sapiens
S-COMT soluble enzyme form Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 MB-COMT protein is quite stable at 37°C Homo sapiens
37
-
 S-COMT protein is quite stable at 37°C Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.035
-
 4-hydroxyestradiol S-COMT, 0.1 mM S-adenosyl-L-methionine Homo sapiens
0.04
-
 norepinephrine S-COMT, 0.1 mM S-adenosyl-L-methionine Homo sapiens
0.077
-
 4-hydroxyestradiol MB-COMT, 0.1 mM S-adenosyl-L-methionine Homo sapiens
0.085
-
 2-hydroxyestradiol MB-COMT, 0.1 mM S-adenosyl-L-methionine Homo sapiens
0.085
-
 epinephrine S-COMT, 0.1 mM S-adenosyl-L-methionine Homo sapiens
0.088
-
 dopamine S-COMT, 0.1 mM S-adenosyl-L-methionine Homo sapiens
0.17
-
 epicatechin S-COMT, 0.25 mM S-adenosyl-L-methionine Homo sapiens
0.18
-
 norepinephrine MB-COMT, 0.1 mM S-adenosyl-L-methionine Homo sapiens
0.18
-
 catechin S-COMT, 0.25 mM S-adenosyl-L-methionine Homo sapiens
0.19
-
 dopamine MB-COMT, 0.1 mM S-adenosyl-L-methionine Homo sapiens
0.25
-
 epinephrine MB-COMT, 0.1 mM S-adenosyl-L-methionine Homo sapiens
0.26
-
 catechin MB-COMT, 0.25 mM S-adenosyl-L-methionine Homo sapiens
0.315
-
 epicatechin MB-COMT, 0.25 mM S-adenosyl-L-methionine Homo sapiens
0.99
-
 2-hydroxyestradiol S-COMT, 0.1 mM S-adenosyl-L-methionine Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8 methylation of 2-hydroxyestradiol or 4-hydroxyestradiol by MB-COMT Homo sapiens
7 8 methylation of 2-hydroxyestradiol or 4-hydroxyestradiol by S-COMT Homo sapiens