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Literature summary for 2.1.1.59 extracted from

  • Paik, W.K.; DiMaria, P.
    Enzymatic methylation and demethylation of protein-bound lysine residues (1984), Methods Enzymol., 106, 274-287.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
S-adenosyl-L-homocysteine competitive versus S-adenosyl-L-methionine Neurospora crassa
S-adenosyl-L-methionine
-
Neurospora crassa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
CNBr peptide residue 1-80 of horse heart cytochrome c Neurospora crassa
0.03
-
[cytochrome c]-L-lysine of horse heart, heme-free Neurospora crassa
0.04
-
CNBr peptide residue 1-65 or residue 66-104 of horse heart cytochrome c Neurospora crassa
0.2
-
[cytochrome c]-L-lysine of horse heart, ethanol-denatured Neurospora crassa
0.32
-
[cytochrome c]-L-lysine of horse heart, native Neurospora crassa
1.7
-
[cytochrome c]-L-lysine
-
Neurospora crassa
19
-
S-adenosyl-L-methionine
-
Neurospora crassa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Neurospora crassa 5829
-
additional information overview Neurospora crassa
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
gel filtration Neurospora crassa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + cytochrome c L-lysine Neurospora crassa
-
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
-
?

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Neurospora crassa

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Neurospora crassa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0295
-
purified enzyme Neurospora crassa

Storage Stability

Storage Stability Organism
0-4°C, 50% saturated ammonium sulfate, stable for several months Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + CNBr peptide
-
Neurospora crassa ?
-
?
S-adenosyl-L-methionine + cytochrome c L-lysine
-
Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
-
?
S-adenosyl-L-methionine + cytochrome c L-lysine horse heart cytochrome c-72 Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine a single lysine residue at position 72 of horse heart cytochrome c is methylated ?
S-adenosyl-L-methionine + cytochrome c L-lysine horse heart cytochrome c-72 Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4 ?
S-adenosyl-L-methionine + cytochrome c L-lysine also CNBr peptides of horse heart cytochrome c can serve as substrates, overview Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine a single lysine residue at position 72 of horse heart cytochrome c is methylated ?
S-adenosyl-L-methionine + cytochrome c L-lysine also CNBr peptides of horse heart cytochrome c can serve as substrates, overview Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4 ?
S-adenosyl-L-methionine + [cytochrome c]-L-lysine
-
Neurospora crassa S-adenosyl-L-homocysteine + [cytochrome c]-N6-methyl-L-lysine
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Neurospora crassa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
assay at Neurospora crassa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.002
-
S-adenosyl-L-methionine
-
Neurospora crassa