Activating Compound | Comment | Organism | Structure |
---|---|---|---|
nsp10 protein | the methyltransferase is only active in the presence of its activating partner nsp10 | Severe acute respiratory syndrome-related coronavirus |
Protein Variants | Comment | Organism |
---|---|---|
D106A | the mutant shows 38% of wild type activity | Severe acute respiratory syndrome-related coronavirus |
D130A | the mutant shows 2% of wild type activity | Severe acute respiratory syndrome-related coronavirus |
D99A | inactive | Severe acute respiratory syndrome-related coronavirus |
E203A | inactive | Severe acute respiratory syndrome-related coronavirus |
G73A | the mutant shows 18% of wild type activity | Severe acute respiratory syndrome-related coronavirus |
I40A | the mutant shows 8% of wild type activity | Severe acute respiratory syndrome-related coronavirus |
K170A | inactive | Severe acute respiratory syndrome-related coronavirus |
K46A | the mutant shows 1% of wild type activity | Severe acute respiratory syndrome-related coronavirus |
L244A | inactive | Severe acute respiratory syndrome-related coronavirus |
M247A | inactive | Severe acute respiratory syndrome-related coronavirus |
M41A | the mutant shows 4% of wild type activity | Severe acute respiratory syndrome-related coronavirus |
N43A | the mutant shows 11% of wild type activity | Severe acute respiratory syndrome-related coronavirus |
Q87A | the mutant shows 62% of wild type activity | Severe acute respiratory syndrome-related coronavirus |
R86A | inactive | Severe acute respiratory syndrome-related coronavirus |
S188A | the substitution shows 72% loss of activity with no significant effect on the stability of the nsp10/nsp16 complex | Severe acute respiratory syndrome-related coronavirus |
T48A | the mutant shows 20% of wild type activity | Severe acute respiratory syndrome-related coronavirus |
T54E | the substitution shows 72% loss of activity with no significant effect on the stability of the nsp10/nsp16 complex | Severe acute respiratory syndrome-related coronavirus |
T58A | the substitution shows 43% loss of activity with no significant effect on the stability of the nsp10/nsp16 complex | Severe acute respiratory syndrome-related coronavirus |
T58E | the substitution shows 99% loss of activity with 54% association of the Nsp10/nsp16 complex compared to the wild type | Severe acute respiratory syndrome-related coronavirus |
T58N | the substitution shows 70% loss of activity with no significant effect on the stability of the nsp10/nsp16 complex | Severe acute respiratory syndrome-related coronavirus |
V104G | the mutant shows 4% of wild type activity | Severe acute respiratory syndrome-related coronavirus |
V44A | the mutant shows 1% of wild type activity | Severe acute respiratory syndrome-related coronavirus |
V78A | inactive | Severe acute respiratory syndrome-related coronavirus |
Y132A | the mutant shows 5% of wild type activity | Severe acute respiratory syndrome-related coronavirus |
Y132F | the mutant shows 9% of wild type activity | Severe acute respiratory syndrome-related coronavirus |
Y132H | inactive | Severe acute respiratory syndrome-related coronavirus |
Y132T | the mutant shows 5% of wild type activity | Severe acute respiratory syndrome-related coronavirus |
Y30A | the mutant shows 1% of wild type activity | Severe acute respiratory syndrome-related coronavirus |
Y30F | the mutant shows 6% of wild type activity | Severe acute respiratory syndrome-related coronavirus |
Y96A | the mutation abrogates nsp10/nsp16 interaction with enzyme activity | Severe acute respiratory syndrome-related coronavirus |
Y96F | the mutation increases both nsp10/nsp16 interaction with MTase activity and enzyme activity | Severe acute respiratory syndrome-related coronavirus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | contains Mg2+, Mg2+ can be substituted by Mn2+ or Ca2+, but not Zn2+ | Severe acute respiratory syndrome-related coronavirus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Severe acute respiratory syndrome-related coronavirus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + m7G(5')pppA-RNA | - |
Severe acute respiratory syndrome-related coronavirus | S-adenosyl-L-homocysteine + m7G(5')pppAm-RNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
(nucleoside-2'-O-)-methyltransferase | - |
Severe acute respiratory syndrome-related coronavirus |
2'-O-MTase | - |
Severe acute respiratory syndrome-related coronavirus |
nsp10/16 complex | - |
Severe acute respiratory syndrome-related coronavirus |
nsp16 protein | - |
Severe acute respiratory syndrome-related coronavirus |
RNA cap 2'-O-methyltransferase | - |
Severe acute respiratory syndrome-related coronavirus |