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Literature summary for 2.1.1.57 extracted from

  • Langberg, S.R.; Moss, B.
    Post-transcriptional modifications of mRNA. Purification and characterization of cap I and cap II RNA (nucleoside-2-)-methyltransferases from HeLa cells (1981), J. Biol. Chem., 256, 10054-10060.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
S-adenosyl-L-homocysteine cap I-methyltransferase; strong Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0000014
-
G(5')pppA-poly(A)
-
Homo sapiens
0.0000016
-
m7G(5')pppA-poly(A)
-
Homo sapiens
0.0006
-
S-adenosyl-L-methionine
-
Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm cap I- and cap II-methyltransferase Homo sapiens 5737
-
nucleus cap I-methyltransferase Homo sapiens 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
KCl activation, 140-180 mM, cap I-methyltransferase Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HeLa-cells, phosphocellulose chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + G(5')pppA-poly(A)
-
Homo sapiens S-adenosyl-L-homocysteine + G(5')pppA-2'-OMe-poly(A)
-
r
S-adenosyl-L-methionine + m7G(5')pppA-poly(A)
-
Homo sapiens S-adenosyl-L-homocysteine + m7G(5')pppA-2'-OMe-poly(A)
-
r
S-adenosyl-L-methionine + m7G(5')pppNmpN-RNA N: purin and pyrimidine nucleotides are methylated, enzyme: cap II-methyltransferase Homo sapiens S-adenosyl-L-homocysteine + m7G(5')pppNmpNm-RNA i.e. cap II ?
S-adenosyl-L-methionine + m7G(5')pppNpN-RNA i.e. cap O, N: purine and pyrimidine nucleotides are methylated, enzyme: cap I-methyltransferase, substrates are RNAs with a capped terminus with at least 2 additional nucleotides, G(5')pppNpNp Homo sapiens S-adenosyl-L-homocysteine + m7G(5')pppNmpN-RNA i.e. cap I ?
S-adenosyl-L-methionine + m7G(5')pppR-RNA satellite tobacco necrosis virus Homo sapiens S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA
-
?
S-adenosyl-L-methionine + m7G(5')pppR-RNA unmethylated vaccinia virus mRNA Homo sapiens S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA
-
?
S-adenosyl-L-methionine + m7G(5')pppR-RNA best substrates are poly(A)-nucleosides and poly-(I)-nucleosides with m7G(5')pppN-ends Homo sapiens S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA
-
?
S-adenosyl-L-methionine + m7GpppApGp i.e. capped dinucleotide, cap I-methyltransferase Homo sapiens S-adenosyl-L-homocysteine + m7GpppAmpGp
-
?
S-adenosyl-L-methionine + m7GpppApGpUp i.e. capped trinucleotide, cap I-methyltransferase Homo sapiens S-adenosyl-L-homocysteine + m7GpppAmpGpUp
-
?
S-adenosyl-L-methionine + mononucleotide such as m7GpppA or GpppA, poor substrates for cap I-methyltransferase Homo sapiens ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
10 min stable Homo sapiens
44
-
and above, inactivation after 20 min Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
beginning from pH 6.9 continous increase of activity Homo sapiens