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Literature summary for 2.1.1.56 extracted from

  • Chen, Y.; Tao, J.; Sun, Y.; Wu, A.; Su, C.; Gao, G.; Cai, H.; Qiu, S., Wu, Y.; Ahola, T., Guo, D.
    Structure-function analysis of severe acute respiratory syndrome coronavirus RNA cap guanine-N7-methyltransferase (2013), J. Virol., 87, 6296-6305.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
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Severe acute respiratory syndrome-related coronavirus

Crystallization (Commentary)

Crystallization (Comment) Organism
structure-function relationships of the N7-MTase. The ExoN domain is closely involved in the activity of the N7-MTase. Two of the 12 critical residues essential for the N7-MTase are located at the N terminus of the core ExoN domain, reinforcing a role of the ExoN domain in the N7-MTase activity of nsp14. The other 10 critical residues are distributed throughout the N7-MTase domain but localized mainly in the S-adenosyl-L-methionine-binding pocket Severe acute respiratory syndrome-related coronavirus

Protein Variants

Protein Variants Comment Organism
C382Y 72% of wild-type methylation activity Severe acute respiratory syndrome-related coronavirus
C414R complete loss of methylation activity Severe acute respiratory syndrome-related coronavirus
D243A 79% of wild-type methylation activity Severe acute respiratory syndrome-related coronavirus
D273A 97% of wild-type methylation activity Severe acute respiratory syndrome-related coronavirus
D331A complete loss of methylation activity Severe acute respiratory syndrome-related coronavirus
D331E 70% of wild-type methylation activity Severe acute respiratory syndrome-related coronavirus
D331Y complete loss of methylation activity Severe acute respiratory syndrome-related coronavirus
D352A 47% of wild-type methylation activity Severe acute respiratory syndrome-related coronavirus
D90A/E92A 93% of wild-type methylation activity Severe acute respiratory syndrome-related coronavirus
F73A 91% of wild-type methylation activity Severe acute respiratory syndrome-related coronavirus
G333A complete loss of methylation activity Severe acute respiratory syndrome-related coronavirus
G416R 45% of wild-type methylation activity Severe acute respiratory syndrome-related coronavirus
H268L 85% of wild-type methylation activity Severe acute respiratory syndrome-related coronavirus
I332A 83% of wild-type methylation activity Severe acute respiratory syndrome-related coronavirus
K336A 50% of wild-type methylation activity Severe acute respiratory syndrome-related coronavirus
K61A 97% of wild-type methylation activity Severe acute respiratory syndrome-related coronavirus
L419A complete loss of methylation activity Severe acute respiratory syndrome-related coronavirus
N256A 97% of wild-type methylation activity Severe acute respiratory syndrome-related coronavirus
N334A 89% of wild-type methylation activity Severe acute respiratory syndrome-related coronavirus
N388A 83% of wild-type methylation activity Severe acute respiratory syndrome-related coronavirus
P335A 61% of wild-type methylation activity Severe acute respiratory syndrome-related coronavirus
R310A complete loss of methylation activity Severe acute respiratory syndrome-related coronavirus
R84A 6% of wild-type methylation activity Severe acute respiratory syndrome-related coronavirus
T428A 27% of wild-type methylation activity Severe acute respiratory syndrome-related coronavirus
W86A complete loss of methylation activity Severe acute respiratory syndrome-related coronavirus
Y368A complete loss of methylation activity Severe acute respiratory syndrome-related coronavirus
Y420A 5% of wild-type methylation activity Severe acute respiratory syndrome-related coronavirus

Organism

Organism UniProt Comment Textmining
Severe acute respiratory syndrome-related coronavirus P0C6X7
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