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Literature summary for 2.1.1.56 extracted from

  • Chung, K.Y.; Dong, H.; Chao, A.T.; Shi, P.Y.; Lescar, J.; Lim, S.P.
    Higher catalytic efficiency of N-7-methylation is responsible for processive N-7 and 2-O methyltransferase activity in dengue virus (2010), Virology, 402, 52-60.
    View publication on PubMed
Search for more literature for 2.1.1.56

Inhibitors

Inhibitors Comment Organism Structure
S-adenosyl-homocysteine
-
 Dengue virus
sinefungin
-
 Dengue virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
 S-adenosyl-L-methionine pH 7.5, 20°C, Michaelis constants for S-adenosyl-methionine (AdoMet) in both reactions are about 10fold lower than for their respective RNA substrates, suggesting that the rate-limiting steps in methylase reactions are associated with RNA templates Dengue virus
1.8
-
 G(5')pppR-RNA pH 7.5, 20°C, Michaelis constants for S-adenosyl-methionine (AdoMet) in both reactions are about 10fold lower than for their respective RNA substrates, suggesting that the rate-limiting steps in methylase reactions are associated with RNA templates Dengue virus

Organism

Organism UniProt Comment Textmining
Dengue virus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + G(5')pppR-RNA
-
 Dengue virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
 ?

Synonyms

Synonyms Comment Organism
N-7-MTase
-
 Dengue virus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000054
-
 S-adenosyl-L-methionine pH 7.5, 20°C Dengue virus
0.0001947
-
 G(5')pppR-RNA pH 7.5, 20°C Dengue virus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00003
-
 pH 7.5, 20°C Dengue virus sinefungin
0.00177
-
 pH 7.5, 20°C Dengue virus S-adenosyl-homocysteine