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Literature summary for 2.1.1.56 extracted from

  • Hall, M.P.; Ho, C.K.
    Characterization of a Trypanosoma brucei RNA cap (guanine N-7) methyltransferase (2006), RNA, 12, 488-497.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.56

Application

Application Comment Organism
analysis second Trypanosoma brucei RNA (guanine N-7) cap methyltransferase, named TbCgm1, TbCmt1 is homologous with TbCgm1, consisting of a C-terminal (guanine N-7) methyltransferase domain and an N-terminal guanylyltransferase domain, which contains signature motifs found in the nucleotidyl transferase superfamily Trypanosoma brucei

Cloned(Commentary)

Cloned (Comment) Organism
TbCmt1 inserted into an inducible T7 RNA polymerase-based pET vector and introduced into Escherichia coli BL21(DE3)Gold Trypanosoma brucei

Protein Variants

Protein Variants Comment Organism
D144A TbCmt1 mutant shows 5% of wild-type activity Trypanosoma brucei
D80A TbCmt1 mutant is unable to support methylation at levels of input protein sufficient to convert all the input substrate to the methylated form by wild-type TbCmt1, specific activity is less than 0.1% of the wild-type activity Trypanosoma brucei
E258A TbCmt1 mutant is unable to support methylation at levels of input protein sufficient to convert all the input substrate to the methylated form by wild-type TbCmt1, specific activity is less than 0.1% of the wild-type activity Trypanosoma brucei
F171A TbCmt1 mutant displays near-wild-type activity Trypanosoma brucei
H174A TbCmt1 mutant displays near-wild-type activity Trypanosoma brucei
K83A TbCmt1 mutant is unable to support methylation at levels of input protein sufficient to convert all the input substrate to the methylated form by wild-type TbCmt1, specific activity is less than 0.1% of the wild-type activity Trypanosoma brucei
V241A TbCmt1 mutant displays near-wild-type activity Trypanosoma brucei
V242A TbCmt1 mutant displays near-wild-type activity Trypanosoma brucei
Y175A TbCmt1 mutant shows 3% of wild-type activity Trypanosoma brucei
Y246A TbCmt1 mutant shows 2% of wild-type activity Trypanosoma brucei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 x * 39000, SDS-PAGE of His-tagged TbCmt1 Trypanosoma brucei

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
TbCmt1 protein purified from soluble extract by nickel-agarose, followed by phosphocellulose chromatography Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + G(5')pppN TbCmt1 Trypanosoma brucei S-adenosyl-L-homocysteine + m7G(5')pppG
-
 ?
S-adenosyl-L-methionine + GpppA TbCmt1 Trypanosoma brucei S-adenosyl-L-homocysteine + m7GpppA
-
 ?
S-adenosyl-L-methionine + GpppG TbCmt1 Trypanosoma brucei S-adenosyl-L-homocysteine + m7GpppG
-
 ?

Subunits

Subunits Comment Organism
? x * 39000, SDS-PAGE of His-tagged TbCmt1 Trypanosoma brucei

Synonyms

Synonyms Comment Organism
RNA cap (guanine N-7) methyltransferase
-
 Trypanosoma brucei
TbCgm1
-
 Trypanosoma brucei
TbCmt1
-
 Trypanosoma brucei