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Literature summary for 2.1.1.56 extracted from

  • Mao, X.; Shuman, S.
    Intrinsic RNA (guanine-7) methyltransferase activity of the vaccinia virus capping enzyme D1 subunit is stimulated by the D12 subunit. Identification of amino acid residues in the D1 protein required for subunit association and methyl group transfer (1994), J. Biol. Chem., 269, 24472-24479.
    View publication on PubMed
Search for more literature for 2.1.1.56

Cloned(Commentary)

Cloned (Comment) Organism
D1 fragment, amino acids 520-844 Vaccinia virus
individual and coexpression of His-tagged D1 large subunit fragment, being the methyltransferase domain of the enzyme, and of D12 small subunit in Escherichia coli Vaccinia virus

Protein Variants

Protein Variants Comment Organism
additional information exchange for alanine at single sites within the methyltransferase domain fragment of D1, loss of activity when exchanged hits His682 and Tyr683, active site Vaccinia virus

Organism

Organism UniProt Comment Textmining
Vaccinia virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
methyltransferase domain, and small subunit of mRNA capping enzyme, recombinant from E. coli Vaccinia virus

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] subunit interaction in vitro and in vivo is a necessary condition for full enzyme activity Vaccinia virus
a
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] RNA triphosphatase, RNA guanylyltransferase, and RNA (guanine-N7-)methyltransferase form the mRNA capping enzyme complex consiting of a small and a large subunit Vaccinia virus
a
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] active site is located at His682 and Tyr683 Vaccinia virus
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Vaccinia virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information isolated methyltransferase domain shows a lower but kinetically equivalent activity than the complete enzyme, which is highly enhanced by association with the D12 subunit Vaccinia virus ?
-
 ?
S-adenosyl-L-methionine + G(5')pppR-RNA
-
 Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
 ?

Subunits

Subunits Comment Organism
More isolated methyltransferase domain shows a lower but kinetically equivalent activity than the complete enzyme, which is highly enhanced by association with the D12 subunit Vaccinia virus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Vaccinia virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Vaccinia virus