Cloned (Comment) | Organism |
---|---|
D1 fragment, amino acids 498 to 844 | Vaccinia virus |
individual and coexpression of His-tagged D1 large subunit fragment, being the methyltransferase domain of the enzyme, and of D12 small subunit in Escherichia coli | Vaccinia virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | strong | Vaccinia virus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
S-adenosyl-L-methionine | intact mRNA capping enzyme and isolated methyltransferase domain | Vaccinia virus | |
0.033 | - |
GTP | intact mRNA capping enzyme and isolated methyltransferase domain | Vaccinia virus | |
0.036 | - |
GTP | isolated methyltransferase domain | Vaccinia virus | |
0.085 | - |
G(5')pppG | intact mRNA capping enzyme | Vaccinia virus | |
0.101 | - |
G(5')pppG | isolated methyltransferase domain | Vaccinia virus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
small subunit of mRNA capping enzyme , gel filtration, SDS-PAGE | Vaccinia virus |
97000 | - |
large subunit of mRNA capping enzyme, gel filtration, SDS-PAGE | Vaccinia virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vaccinia virus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
methyltransferase domain, and small subunit of mRNA capping enzyme, recombinant from E. coli | Vaccinia virus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] | RNA triphosphatase, RNA guanylyltransferase, and RNA (guanine-N7-)methyltransferase form the mRNA capping enzyme complex consiting of a small and a large subunit | Vaccinia virus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0196 | - |
purified D1 fragment, residues 498-844 | Vaccinia virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | isolated methyltransferase domain shows a lower but kinetically equivalent activity than the complete enzyme, which is highly enhanced by association with the D12 subunit | Vaccinia virus | ? | - |
? | |
S-adenosyl-L-methionine + G(5')pppR-RNA | - |
Vaccinia virus | S-adenosyl-L-homocysteine + m7G(5')pppR-RNA | - |
? | |
S-adenosyl-L-methionine + GpppG | - |
Vaccinia virus | S-adenosyl-L-homocysteine + m7GpppG | - |
? | |
S-adenosyl-L-methionine + GTP | - |
Vaccinia virus | S-adenosyl-L-homocysteine + m7GTP | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | isolated methyltransferase domain shows a lower but kinetically equivalent activity than the complete enzyme, which is highly enhanced by association with the D12 subunit | Vaccinia virus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Vaccinia virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Vaccinia virus |