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Literature summary for 2.1.1.53 extracted from

  • Biastoff, S.; Reinhardt, N.; Reva, V.; Brandt, W.; Draeger, B.
    Evolution of putrescine N-methyltransferase from spermidine synthase demanded alterations in substrate binding (2009), FEBS Lett., 583, 3367-3374.
    View publication on PubMed
Search for more literature for 2.1.1.53

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli M15[pREP4] cells Datura stramonium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0561
-
 S-adenosyl-L-methionine wild type enzyme, in 20 mM HEPES, pH 8.0, 2 mM dithiothreitol, 1 mM ascorbic acid, at 37°C Datura stramonium
0.0758
-
 putrescine SPDS-PMT chimeric enzyme consisting of 144 N-terminal amino acids of spermidine synthase and 162 C-terminal amino acids of putrescine N-methyltransferase, in 20 mM HEPES, pH 8.0, 2 mM dithiothreitol, 1 mM ascorbic acid, at 37°C Datura stramonium
0.2054
-
 putrescine wild type enzyme, in 20 mM HEPES, pH 8.0, 2 mM dithiothreitol, 1 mM ascorbic acid, at 37°C Datura stramonium
0.5299
-
 putrescine PMT-SPDS chimeric enzyme consisting of 181 N-terminal amino acids of putrescine N-methyltransferase and 165 C-terminal amino acids of spermidine synthase, in 20 mM HEPES, pH 8.0, 2 mM dithiothreitol, 1 mM ascorbic acid, at 37°C Datura stramonium

Organism

Organism UniProt Comment Textmining
Datura stramonium Q70EW6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + putrescine
-
 Datura stramonium S-adenosyl-L-homocysteine + N-methylputrescine
-
 ?

Subunits

Subunits Comment Organism
homodimer gel filtration Datura stramonium

Synonyms

Synonyms Comment Organism
PMT
-
 Datura stramonium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.4
-
 putrescine PMT-SPDS chimeric enzyme consisting of 181 N-terminal amino acids of putrescine N-methyltransferase and 165 C-terminal amino acids of spermidine synthase, in 20 mM HEPES, pH 8.0, 2 mM dithiothreitol, 1 mM ascorbic acid, at 37°C Datura stramonium
0.69
-
 putrescine SPDS-PMT chimeric enzyme consisting of 144 N-terminal amino acids of spermidine synthase and 162 C-terminal amino acids of putrescine N-methyltransferase, in 20 mM HEPES, pH 8.0, 2 mM dithiothreitol, 1 mM ascorbic acid, at 37°C Datura stramonium
5.14
-
 putrescine wild type enzyme, in 20 mM HEPES, pH 8.0, 2 mM dithiothreitol, 1 mM ascorbic acid, at 37°C Datura stramonium