Application | Comment | Organism |
---|---|---|
analysis | convenient and accurate enzyme-coupled colorimetric PMT assay, based on the conversion of S-adenosyl-L-homocysteine by 5'-methylthioacenosine/S-adenosylhomocysteine nucleosidase and S-ribosylhomocysteine lyase | Datura stramonium |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta-gami (DE3), transformed with a pET-21d vector encoding pmt | Datura stramonium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-homocysteine | - |
Datura stramonium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Datura stramonium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by Ni2+-chelate affinity chromatography | Datura stramonium |
Storage Stability | Organism |
---|---|
4°C, glycine-NaOH buffer, pH 9, stable for 1 month | Datura stramonium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + putrescine | - |
Datura stramonium | S-adenosyl-L-homocysteine + N-methylputrescine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PMT | - |
Datura stramonium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Datura stramonium |