Application | Comment | Organism |
---|---|---|
pharmacology | Catharanthus roseus is the sole commercial source for monoterpenoid indole alkaloids vindoline and catharanthine, components of the anticancer dimers vinblastine and vincristine | Catharanthus roseus |
Cloned (Comment) | Organism |
---|---|
PCR-amplification of cDNA, insertion into expression vector pET 30b, ligated into pGEM-TEasy, heat shock transformation of Escherichia coli DE3-MRF | Catharanthus roseus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
loganin | reaction product | Catharanthus roseus | |
S-adenosyl-L-homocysteine | - |
Catharanthus roseus | |
S-adenosyl-L-methionine | - |
Catharanthus roseus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14.76 | - |
loganic acid | pH 7.5, 37°C | Catharanthus roseus | |
742.1 | - |
S-adenosyl-L-methionine | pH 7.5, 37°C | Catharanthus roseus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42020 | - |
calculated from 371-amino acid open reading frame | Catharanthus roseus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
loganic acid + S-adenosyl-L-methionine | Catharanthus roseus | - |
loganin + S-adenosyl-L-homocysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Catharanthus roseus | B2KPR3 | cultivar Little Delicata, Madagaskar periwinkle | - |
Purification (Comment) | Organism |
---|---|
crude recombinant enzyme, for enzyme activity location study upper and lower epidermis of leaves is abraded with carborundum, leaves rubbed with cotton swabs and then dipped in Trizol at 4°C and agitated | Catharanthus roseus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epidermis | - |
Catharanthus roseus | - |
flower | highest activity | Catharanthus roseus | - |
leaf | 10fold higher enzyme activity in epidermis extracts than in whole leaves, 50% lower activity in aged L2, L3, and L4 leaves compared to young L1 leaves | Catharanthus roseus | - |
root | 4-8times lower activity than in the other tested plant organs | Catharanthus roseus | - |
silique | similar activity as in leaves | Catharanthus roseus | - |
stem | second highest activity | Catharanthus roseus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000000517 | - |
secologanic acid, buffer (100 mM Tris-HCl, pH 7.5, 14 mM beta-mercaptoethanol, 25 mM KCl), 37°C, 30 min assay | Catharanthus roseus |
0.00000517 | - |
loganic acid, buffer (100 mM Tris-HCl, pH 7.5, 14 mM beta-mercaptoethanol, 25 mM KCl), 37°C, 30 min assay | Catharanthus roseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
loganic acid + S-adenosyl-L-methionine | - |
Catharanthus roseus | loganin + S-adenosyl-L-homocysteine | - |
? | |
secologanic acid + S-adenosyl-L-methionine | 10% activity compared to loganic acid | Catharanthus roseus | secologanin + S-adenosyl-L-homocysteine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LAMT | - |
Catharanthus roseus |
loganic acid O-methyltransferase | - |
Catharanthus roseus |
rLAMT | recombinant LAMT | Catharanthus roseus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.327 | - |
loganic acid | pH 7.5, 37°C | Catharanthus roseus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Catharanthus roseus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | Mg2+ or K+ are not required for enzyme activity nor to enhance it | Catharanthus roseus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
S-adenosyl-L-methionine | pH 7.5, 37°C | Catharanthus roseus | |
0.215 | - |
loganin | pH 7.5, 37°C | Catharanthus roseus | |
0.4 | - |
S-adenosyl-L-homocysteine | pH 7.5, 37°C | Catharanthus roseus |
General Information | Comment | Organism |
---|---|---|
metabolism | enzyme in the monoterpenoid indole alkaloid biosynthesis pathway | Catharanthus roseus |