Cloned (Comment) | Organism |
---|---|
expressed in COS-1 cells and in Escherichia coli | Homo sapiens |
impossible to express BHMT2 in mammalian cells, protein aggregates after bacterial expression. BHMT2 is rapidly degraded in a rabbit reticulocyte lysate, but it can be stabilized by cotransfection of COS-1 cells with BHMT and, after cotransfection, it coprecipitates with BHMT | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A66V | nonsynonymous SNP identified using 240 DNA samples from four ethnic groups | Homo sapiens |
Arg16Cys | nonsynonymous SNP identified using 240 DNA samples from four ethnic groups, Km (mM): 0.0139 (betaine), 0.0076 (L-homocysteine) | Homo sapiens |
Arg239Gln | nonsynonymous SNP identified using 240 DNA samples from four ethnic groups, Km (mM): 0.012 (betaine), 0.0158 (L-homocysteine) | Homo sapiens |
Gly199Ser | nonsynonymous SNP identified using 240 DNA samples from four ethnic groups, Km (mM): 0.0139 (betaine), 0.0206 (L-homocysteine) | Homo sapiens |
Pro197Ser | nonsynonymous SNP identified using 240 DNA samples from four ethnic groups, Km (mM): 0.0213 (betaine), 0.0216 (L-homocysteine) | Homo sapiens |
T218M | nonsynonymous SNP identified using 240 DNA samples from four ethnic groups | Homo sapiens |
V155F | nonsynonymous SNP identified using 240 DNA samples from four ethnic groups | Homo sapiens |
V237M | nonsynonymous SNP identified using 240 DNA samples from four ethnic groups | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0076 | - |
L-homocysteine | mutant Arg16Cys | Homo sapiens | |
0.012 | - |
betaine | mutant Arg239Gln | Homo sapiens | |
0.0139 | - |
betaine | mutant Arg16Cys | Homo sapiens | |
0.0139 | - |
betaine | mutant Gly199Ser | Homo sapiens | |
0.0158 | - |
L-homocysteine | mutant Arg239Gln | Homo sapiens | |
0.0206 | - |
L-homocysteine | mutant Gly199Ser | Homo sapiens | |
0.0213 | - |
betaine | mutant Pro197Ser | Homo sapiens | |
0.0216 | - |
L-homocysteine | mutant Pro197Ser | Homo sapiens | |
0.0227 | - |
betaine | wild-type | Homo sapiens | |
0.029 | - |
L-homocysteine | wild-type | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | no expression of BHMT2 protein is detected in liver or kidney although BHMT2 mRNA expression is observed | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-homocysteine + betaine | - |
Homo sapiens | L-methionine + dimethylglycine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
betaine-homocysteine S-methyltransferase | - |
Homo sapiens |
BHMT | - |
Homo sapiens |
BHMT2 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |