Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Shrimp white spot syndrome virus |
expressed in Escherichia coli BL21(DE3) cells | Penaeus vannamei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(S)-2-(5(((1,2-dihydro-3-methyl-1-oxobenzo[f]quinazolin-9 yl)methyl)amino)1-oxo-2-isoindolinyl)glutaric acid | - |
Penaeus vannamei | |
(S)-2-(5(((1,2-dihydro-3-methyl-1-oxobenzo[f]quinazolin-9-yl)methyl)amino)1-oxo-2-isoindolinyl)glutaric acid | - |
Shrimp white spot syndrome virus | |
10-propargyl-5,8-dideazafolate | - |
Penaeus vannamei | |
10-propargyl-5,8-dideazafolate | - |
Shrimp white spot syndrome virus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
dUMP | at 30°C | Shrimp white spot syndrome virus | |
0.0021 | - |
dUMP | at 30°C | Penaeus vannamei | |
0.0134 | - |
5,10-methylenetetrahydrofolate | at 30°C | Shrimp white spot syndrome virus | |
0.0135 | - |
5,10-methylenetetrahydrofolate | at 30°C | Penaeus vannamei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penaeus vannamei | C6GJB8 | - |
- |
Shrimp white spot syndrome virus | Q8QTF3 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and Q-Sepharose column chromatography | Shrimp white spot syndrome virus |
ammonium sulfate precipitation and Q-Sepharose column chromatography | Penaeus vannamei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | - |
Shrimp white spot syndrome virus | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Penaeus vannamei | dihydrofolate + dTMP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ThyA | - |
Shrimp white spot syndrome virus |
ThyA | - |
Penaeus vannamei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
5,10-methylenetetrahydrofolate | at 30°C | Shrimp white spot syndrome virus | |
4.1 | - |
5,10-methylenetetrahydrofolate | at 30°C | Penaeus vannamei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
N5,N10-methylenetetrahydrofolate | - |
Shrimp white spot syndrome virus | |
N5,N10-methylenetetrahydrofolate | - |
Penaeus vannamei |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00015 | - |
at 30°C | Penaeus vannamei | (S)-2-(5(((1,2-dihydro-3-methyl-1-oxobenzo[f]quinazolin-9 yl)methyl)amino)1-oxo-2-isoindolinyl)glutaric acid | |
0.00017 | - |
at 30°C | Penaeus vannamei | 10-propargyl-5,8-dideazafolate | |
0.00019 | - |
at 30°C | Shrimp white spot syndrome virus | (S)-2-(5(((1,2-dihydro-3-methyl-1-oxobenzo[f]quinazolin-9 yl)methyl)amino)1-oxo-2-isoindolinyl)glutaric acid | |
0.00023 | - |
at 30°C | Shrimp white spot syndrome virus | 10-propargyl-5,8-dideazafolate |
General Information | Comment | Organism |
---|---|---|
physiological function | thymidylate synthase is the only de novo source of dTMP and is essential for DNA replication | Shrimp white spot syndrome virus |
physiological function | thymidylate synthase is the only de novo source of dTMP and is essential for DNA replication | Penaeus vannamei |