Application | Comment | Organism |
---|---|---|
medicine | Lovo 175X2 cells transfected with mutant tumor suppressor gene p53 are more resistant to 5-fluorouracil, nolatrexed, raltitrexed and pemetrexed in comparison with the wild-type p53 parental cells Lovo 92. Resistance is associated with an increase in thymidylate synthase protein expression and catalytic activity. Lovo li cells, characterized by functionally inactive p53, are 3-13fold more sensitive to nolatrexed, raltitrexed and pemetrexed, and have a lower thymidylate synthase mRNA, protein expression and catalytic activity than Lovo 92. Mutant p53 WiDr cells transfected with wt p53 are not significantly different from mutant p53 WiDr cells with respect to sensitivity to thymidylate synthase inhibitors or thymidylate synthase levels | Homo sapiens |
medicine | pharmacological treatment of colorectal cancer | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-fluorouracil | but a major mechanism of resistance to 5-fluorouracil is overexpression of thymidylate synthase | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | Homo sapiens | - |
7,8-dihydrofolate + dTMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
LoVo cell | - |
Homo sapiens | - |
Lovo-175X2 cell | - |
Homo sapiens | - |
Lovo-92 cell | human colon carcinoma cell line | Homo sapiens | - |
WiDr cell | - |
Homo sapiens | - |
WiDr cell | human colon carcinoma cell line | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | - |
Homo sapiens | 7,8-dihydrofolate + dTMP | - |
? |