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Literature summary for 2.1.1.45 extracted from
- Cloning, expression and characterization of Babesia gibsoni dihydrofolate reductase-thymidylate synthase: inhibitory effect of antifolates on its catalytic activity and the parasite proliferation (2008), Antimicrob. Agents Chemother., 52, 4072-4080.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Babesia gibsoni |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| antifolate | inhibits activitiy of bifunctional DHFR-TS enzyme | Babesia gibsoni |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Babesia gibsoni | 5737 | - |
| soluble | - |
Babesia gibsoni | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
SDS-PAGE, bifunctional native BgDHFR-TS enzyme from the parasite lysate | Babesia gibsoni |
| 58800 | - |
x * 58800, calculated, 57000, SDS-PAGE | Babesia gibsoni |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + deoxyuridine monophosphate | Babesia gibsoni | - |
7,8-dihydrofolate + deoxythymidine monophosphate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Babesia gibsoni | - |
bifunctional dihydrofolate reductase-thymidylate synthase | - |
| Babesia gibsoni | B5U9U8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| glutathione elusion method | Babesia gibsoni |
| recombinant protein | Babesia gibsoni |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| merozoite | - |
Babesia gibsoni | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + deoxyuridine monophosphate | - |
Babesia gibsoni | 7,8-dihydrofolate + deoxythymidine monophosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 58800, calculated, 57000, SDS-PAGE | Babesia gibsoni |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BgDHFR-TS | - |
Babesia gibsoni |
| DHFR-TS | - |
Babesia gibsoni |
| dihydrofolate reductase-thymidylate synthase | - |
Babesia gibsoni |
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