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Literature summary for 2.1.1.45 extracted from

  • Spencer, H.T.; Villafranca, J.E.; Appleman, J.R.
    Kinetic scheme for thymidylate synthase from Escherichia coli: determination from measurements of ligand binding, primary and secondary isotope effects, and pre-steady-state catalysis (1997), Biochemistry, 36, 4212-4222.
    View publication on PubMed

Application

Application Comment Organism
medicine
-
Mus musculus
medicine enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs Homo sapiens
medicine enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs Lacticaseibacillus casei
medicine enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs Tequatrovirus T4

Cloned(Commentary)

Cloned (Comment) Organism
-
Mus musculus
-
Escherichia coli
-
Homo sapiens
-
Lacticaseibacillus casei
-
Tequatrovirus T4
-
Candida albicans

Inhibitors

Inhibitors Comment Organism Structure
10-propargyl-5,8-dideazafolate
-
Escherichia coli
5-Mercapto-2'-deoxyuridylic acid
-
Homo sapiens
dihydrofolate
-
Escherichia coli
thymidine phosphate
-
Escherichia coli
thymidine phosphate
-
Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
methylenetetrahydrofolate
-
Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
-
Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + dUMP Mus musculus
-
dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Lacticaseibacillus casei
-
dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Tequatrovirus T4
-
dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Candida albicans
-
dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Escherichia coli biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Homo sapiens biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
?

Organism

Organism UniProt Comment Textmining
Candida albicans
-
-
-
Escherichia coli
-
-
-
Homo sapiens
-
-
-
Lacticaseibacillus casei
-
-
-
Mus musculus
-
-
-
Tequatrovirus T4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP
-
Escherichia coli 7,8-dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Mus musculus dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Mus musculus dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Escherichia coli dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Homo sapiens dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Lacticaseibacillus casei dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Lacticaseibacillus casei dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Tequatrovirus T4 dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Tequatrovirus T4 dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Candida albicans dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Candida albicans dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Escherichia coli dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Homo sapiens dihydrofolate + dTMP
-
?
5-bromo-2'-deoxyuridine 5'-monophosphate + ? dehalogenation Mus musculus ?
-
?
5-bromo-2'-deoxyuridine 5'-monophosphate + ? dehalogenation Escherichia coli ?
-
?
5-bromo-2'-deoxyuridine 5'-monophosphate + ? dehalogenation Lacticaseibacillus casei ?
-
?
5-iodo-2'-deoxyuridine 5'-monophosphate + ?
-
Mus musculus ?
-
?
5-iodo-2'-deoxyuridine 5'-monophosphate + ?
-
Escherichia coli ?
-
?

Subunits

Subunits Comment Organism
dimer
-
Mus musculus
dimer
-
Escherichia coli
dimer
-
Homo sapiens
dimer
-
Lacticaseibacillus casei
dimer
-
Tequatrovirus T4

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00001
-
5-Mercapto-2'-deoxyuridylic acid
-
Homo sapiens