Application | Comment | Organism |
---|---|---|
medicine | - |
Mus musculus |
medicine | enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs | Homo sapiens |
medicine | enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs | Lacticaseibacillus casei |
medicine | enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs | Tequatrovirus T4 |
Cloned (Comment) | Organism |
---|---|
- |
Mus musculus |
- |
Escherichia coli |
- |
Homo sapiens |
- |
Lacticaseibacillus casei |
- |
Tequatrovirus T4 |
- |
Candida albicans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
10-propargyl-5,8-dideazafolate | - |
Escherichia coli | |
5-Mercapto-2'-deoxyuridylic acid | - |
Homo sapiens | |
dihydrofolate | - |
Escherichia coli | |
thymidine phosphate | - |
Escherichia coli | |
thymidine phosphate | - |
Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
methylenetetrahydrofolate | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
- |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | Mus musculus | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Lacticaseibacillus casei | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Tequatrovirus T4 | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Candida albicans | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Escherichia coli | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Homo sapiens | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | dihydrofolate + dTMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | - |
- |
- |
Escherichia coli | - |
- |
- |
Homo sapiens | - |
- |
- |
Lacticaseibacillus casei | - |
- |
- |
Mus musculus | - |
- |
- |
Tequatrovirus T4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP | - |
Escherichia coli | 7,8-dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Homo sapiens | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Tequatrovirus T4 | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Tequatrovirus T4 | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Candida albicans | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Candida albicans | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | Escherichia coli | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | Homo sapiens | dihydrofolate + dTMP | - |
? | |
5-bromo-2'-deoxyuridine 5'-monophosphate + ? | dehalogenation | Mus musculus | ? | - |
? | |
5-bromo-2'-deoxyuridine 5'-monophosphate + ? | dehalogenation | Escherichia coli | ? | - |
? | |
5-bromo-2'-deoxyuridine 5'-monophosphate + ? | dehalogenation | Lacticaseibacillus casei | ? | - |
? | |
5-iodo-2'-deoxyuridine 5'-monophosphate + ? | - |
Mus musculus | ? | - |
? | |
5-iodo-2'-deoxyuridine 5'-monophosphate + ? | - |
Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Mus musculus |
dimer | - |
Escherichia coli |
dimer | - |
Homo sapiens |
dimer | - |
Lacticaseibacillus casei |
dimer | - |
Tequatrovirus T4 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00001 | - |
5-Mercapto-2'-deoxyuridylic acid | - |
Homo sapiens |