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Literature summary for 2.1.1.45 extracted from
- A convenient procedure for purification of thymidylate synthase from L1210 cells (1982), Anal. Biochem., 121, 275-280.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | - |
Mus musculus |
| medicine | enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs | Homo sapiens |
| medicine | enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs | Lacticaseibacillus casei |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 10-formyl folic acid | - |
Mus musculus |  |
| 5-fluoro-2'-deoxyuridylic acid | - |
Homo sapiens | |
| 5-fluoro-2'-deoxyuridylic acid | - |
Lacticaseibacillus casei | |
| 5-fluoro-2'-deoxyuridylic acid | - |
Mus musculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
2 * 38000, SDS-PAGE | Mus musculus |
| 38500 | - |
2 * 38500, SDS-PAGE | Mus musculus |
| 58000 | - |
- |
Gallus gallus |
| 76000 | - |
- |
Homo sapiens |
| 76000 | - |
L1210 cells, purified enzyme, gel filtration | Mus musculus |
| 78000 | - |
L1210 cells, gel filtration | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + dUMP | Gallus gallus | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Mus musculus | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Homo sapiens | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Lacticaseibacillus casei | - |
dihydrofolate + dTMP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
chicken | - |
| Homo sapiens | - |
- |
- |
| Lacticaseibacillus casei | - |
- |
- |
| Mus musculus | - |
L1210 cells | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Gallus gallus |
- |
Lacticaseibacillus casei |
| L1210 cells | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| blast cell | from patients with acute myelocytic leukemia | Homo sapiens | - |
| embryo | - |
Gallus gallus | - |
| peritoneum | L1210 leukemia cells grown in the peritoneal cavity of CDF1 mice | Mus musculus | - |
| thymus | - |
Mus musculus | - |
| thymus | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + dUMP | - |
Gallus gallus | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Gallus gallus | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Homo sapiens | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Homo sapiens | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 38500, SDS-PAGE | Mus musculus |
| dimer | 2 * 38000, SDS-PAGE | Mus musculus |
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Release 2023.1 (January 2023)
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