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Literature summary for 2.1.1.41 extracted from
- Purification, characterization and inhibition of sterol C24-methyltransferase from Candida albicans (2011), Arch. Biochem. Biophys., 505, 194-201.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 24(R,S),25-epiminolanosterol | - |
Candida albicans |  |
| 24-thialanosterol | - |
Candida albicans | |
| 25-azalanosterol | - |
Candida albicans | |
| 26,27-dehydrozymosterol | - |
Candida albicans | |
| cholesta-8,24(28)-dienol | - |
Candida albicans | |
| ergosta-5,7,22-trienol | - |
Candida albicans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
Zymosterol | pH 7.5, 30°C | Candida albicans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
SDS-PAGE | Candida albicans |
| 178000 | - |
gel filtration | Candida albicans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida albicans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using anion exchange chromatography, gel filtration and fast performance liquid chromatography using a Mono Q column | Candida albicans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + zymosterol | - |
Candida albicans | S-adenosyl-L-homocysteine + fecosterol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 43000, SDS-PAGE | Candida albicans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 24-SMT | - |
Candida albicans |
| sterol C24-methyltransferase | - |
Candida albicans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Candida albicans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
Zymosterol | pH 7.5, 30°C | Candida albicans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Candida albicans |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000009 | - |
26,27-dehydrozymosterol | pH 7.5, 30°C | Candida albicans | |
| 0.000011 | - |
24(R,S),25-epiminolanosterol | pH 7.5, 30°C | Candida albicans | |
| 0.000054 | - |
25-azalanosterol | pH 7.5, 30°C | Candida albicans | |
| 0.02 | - |
ergosta-5,7,22-trienol | pH 7.5, 30°C | Candida albicans | |
| 0.072 | - |
cholesta-8,24(28)-dienol | pH 7.5, 30°C | Candida albicans | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
pH 7.5, 30°C | Candida albicans | 25-azalanosterol | |
| 0.005 | - |
pH 7.5, 30°C | Candida albicans | 24(R,S),25-epiminolanosterol | |
| 0.02 | - |
pH 7.5, 30°C | Candida albicans | 24-thialanosterol | |
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