Application | Comment | Organism |
---|---|---|
medicine | the results indicate that SMT/MPL-SE can be an effective vaccine candidate for use against visceral leishmaniasis | Leishmania infantum |
medicine | vaccine development, visceral leishmaniasis | Leishmania infantum |
Cloned (Comment) | Organism |
---|---|
expressed as a His-tagged fusion protein in Escherichia coli | Leishmania infantum |
pET-28a vector, expressed in Escherichia coli Rosetta, recombinant protein | Leishmania infantum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | C57BL/6 mice immunized with a vaccine candidate consisting of 24-c-methyltransferase as an antigene formulated monophosphoryl lipid A (MPL-SE) show Ag-specific Th1 immune responses characterized by robust production of IFN-gamma upon specific Ag re-exposure in vitro. Upon challenge with Leishmania infantum, mice immunized with SMT plus MPL-SE show significant lower parasite burdens in both spleens and livers compared with non-immunized mice or mice injected with adjuvant alone | Leishmania infantum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39800 | - |
predicted from sequence, SDS-PAGE | Leishmania infantum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania infantum | - |
- |
- |
Leishmania infantum | A4IDL2 | cloning of SMT protein of | - |
Purification (Comment) | Organism |
---|---|
recombinant protein, SDS-PAGE | Leishmania infantum |
using Ni-NTA-chromatography | Leishmania infantum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
promastigote | - |
Leishmania infantum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
cloning of SMT gene, sequence homologies and Western Blot comparable between Leishmania donovani, Leishmania infantum and Leishmania major, serological evaluation revealed that SMT is recognized by visceral leishmaniasis (VL) patients, immunized mice shown to have lower parasite burdens in both spleens and livers compared with non-immunized mice or mice injected with adjuvant alone | Leishmania infantum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme involved in biosynthesis of ergosterol, which is a target molecule of leishmanicidal and fungicidal amphotericin B, used for vaccine development | Leishmania infantum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SMT | - |
Leishmania infantum |
SMT protein | - |
Leishmania infantum |
sterol 24-C-methyltransferase | - |
Leishmania infantum |