Protein Variants | Comment | Organism |
---|---|---|
C128L | no activity with AdoMet as substrate, Kd (mM) (zymosterol): 0.005, Kd (mM) (AdoMet): 0.028, photolabelled: value below 1% | Saccharomyces cerevisiae |
C128S | Km (mM) (substrate: AdoMet): 30, kcat (1/sec) (substrate: AdoMet): 0.005, Kd (mM) (zymosterol): 0.005, Kd (mM) (AdoMet): 0.008 | Saccharomyces cerevisiae |
D125L | no activity with AdoMet as substrate, Kd (mM) (zymosterol): 0.006, Kd (mM) (AdoMet): 0.012, photolabelled: 30% | Saccharomyces cerevisiae |
D152L | no activity with AdoMet as substrate, Kd (mM) (zymosterol): 0.005, Kd (mM) (AdoMet): 0.014, photolabelled: 35% | Saccharomyces cerevisiae |
additional information | by photoaffinity labeling and mutational analysis the AdoMet binding site is defined. Results indicate that one or both of Cys128 and Pro133 are covalently bound to AdoMet | Saccharomyces cerevisiae |
P133L | no activity with AdoMet as substrate, Kd (mM) (zymosterol): 0.015, Kd (mM) (AdoMet): no binding, photolabelled: value below 1% | Saccharomyces cerevisiae |
Y153F | Km (mM) (substrate: AdoMet): 0.016, kcat (1/sec) (substrate: AdoMet): 0.003, Kd (mM) (zymosterol): 0.007, Kd (mM) (AdoMet): 0.013, photolabelled: 55% | Saccharomyces cerevisiae |
Y153L | Km (mM) (substrate: AdoMet): 0.017, kcat (1/sec) (substrate: AdoMet): 0.01, Kd (mM) (zymosterol): 0.004, Kd (mM) (AdoMet): 0.004 | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-deoxy-5'-(methylthio)adenosine | AdoMet analog, competitive inhibitor | Saccharomyces cerevisiae | |
S-isobutyladenosine | AdoMet analog, competitive inhibitor | Saccharomyces cerevisiae | |
sinefungin | AdoMet analog, competitive inhibitor | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
AdoMet | mutant Tyr153Phe | Saccharomyces cerevisiae | |
0.017 | - |
AdoMet | wild-type | Saccharomyces cerevisiae | |
0.017 | - |
AdoMet | mutant Tyr153Leu | Saccharomyces cerevisiae | |
0.03 | - |
AdoMet | mutant Cys128Ser | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + zymosterol | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + fecosterol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
24-C-sterol methyltransferase | - |
Saccharomyces cerevisiae |
SMT | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
AdoMet | mutant Tyr153Phe | Saccharomyces cerevisiae | |
0.005 | - |
AdoMet | mutant Cys128Ser | Saccharomyces cerevisiae | |
0.01 | - |
AdoMet | wild-type | Saccharomyces cerevisiae | |
0.01 | - |
AdoMet | mutant Tyr153Leu | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
AdoMet | - |
Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
sinefungin | - |
Saccharomyces cerevisiae | |
0.022 | - |
5'-deoxy-5'-(methylthio)adenosine | - |
Saccharomyces cerevisiae | |
0.058 | - |
S-isobutyladenosine | - |
Saccharomyces cerevisiae |