Literature summary for 2.1.1.41 extracted from

Nes, W.D.; Marshall, J.A.; Jia, Z.; Jaradat, T.T.; Song, Z.; Jayasimha, P.
Active site mapping and substrate channeling in the sterol methyltransferase pathway (2002), J. Biol. Chem., 277, 42549-42556.

Search for more literature for 2.1.1.41

Cloned(Commentary)

Cloned (Comment)	Organism
expression of exchange mutants in Escherichia coli	Saccharomyces cerevisiae
overexpression in Escherichia coli	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
D65L	potentially responsible for substrate binding to leucine by site directed mutagenesis, unchanged activity	Saccharomyces cerevisiae
D79L	potentially responsible for substrate binding to leucine by site directed mutagenesis, not decreased activity, 1 new activity to form 24-methylzymosterol from zymosterol	Saccharomyces cerevisiae
E64L	potentially responsible for substrate binding to leucine by site directed mutagenesis, unchanged activity	Saccharomyces cerevisiae
E68L	potentially responsible for substrate binding to leucine by site directed mutagenesis, no activity	Saccharomyces cerevisiae
E82L	potentially responsible for substrate binding to leucine by site directed mutagenesis, not decreased activity, 1 new activity to form 24-methylzymosterol from zymosterol	Saccharomyces cerevisiae
E98L	potentially responsible for substrate binding to leucine by site directed mutagenesis, unchanged activity	Saccharomyces cerevisiae

Inhibitors

Inhibitors	Comment	Organism	Structure
26,27-dehydrozymosterol	mechanism-based irreversible inhibition	Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.013	-	26,27-dehydrozymosterol	mutant E64L	Saccharomyces cerevisiae
0.015	-	26,27-dehydrozymosterol	mutant D65L	Saccharomyces cerevisiae
0.015	0.017	26,27-dehydrozymosterol	wild-type	Saccharomyces cerevisiae
0.017	-	26,27-dehydrozymosterol	mutant D79L	Saccharomyces cerevisiae
0.022	-	26,27-dehydrozymosterol	mutant E82L	Saccharomyces cerevisiae
0.074	-	26,27-dehydrozymosterol	mutant E98L	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
zymosterol + S-adenosyl-L-methionine	Saccharomyces cerevisiae	-	fecosterol + S-adenosyl-L-homocysteine	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant mutant from E. coli	Saccharomyces cerevisiae
recombinant wild-type from E. coli	Saccharomyces cerevisiae

Reaction

Reaction	Comment	Organism	Reaction ID
S-adenosyl-L-methionine + 5alpha-cholesta-8,24-dien-3beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5alpha-cholest-8-en-3beta-ol	substrate analogue inhibitor binding site structure	Saccharomyces cerevisiae	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
26,27-dehydrozymosterol + S-adenosyl-L-methionine	mutants	Saccharomyces cerevisiae	26-homo-cholesta-8(9),23(24)E,26(26')-trienol + 26-homo-cholesta-8(9),26(26')-3beta,24beta-dienol + S-adenosyl-L-homocysteine	-	?
zymosterol + S-adenosyl-L-methionine	-	Saccharomyces cerevisiae	fecosterol + S-adenosyl-L-homocysteine	-	?
zymosterol + S-adenosyl-L-methionine	wild-type and mutants	Saccharomyces cerevisiae	fecosterol + S-adenosyl-L-homocysteine	-	?
zymosterol + S-adenosyl-L-methionine	mutants D79L and E82L	Saccharomyces cerevisiae	24-methylzymosta-8,25(27)-dienol + S-adenosyl-L-homocysteine	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0008	-	26,27-dehydrozymosterol	-	Saccharomyces cerevisiae
0.001	-	Zymosterol	mutant E98L	Saccharomyces cerevisiae
0.005	-	Zymosterol	mutants E82L and E64L	Saccharomyces cerevisiae
0.007	-	Zymosterol	mutant D65L	Saccharomyces cerevisiae
0.01	-	Zymosterol	wild-type and mutant D79L	Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0011	-	26,27-dehydrozymosterol	-	Saccharomyces cerevisiae

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)