Application | Comment | Organism |
---|---|---|
medicine | target for drug development against pneumonitis caused by this pathogen based on its unique substrate preference for lanosterol and 24-methylenelanosterol | Pneumocystis carinii |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, His-tagged protein | Pneumocystis carinii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0106 | - |
lanosterol | recombinant enzyme | Pneumocystis carinii | |
0.019 | - |
24-methylenelanosterol | recombinant enzyme | Pneumocystis carinii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
zymosterol + S-adenosyl-L-methionine | Pneumocystis carinii | - |
fecosterol + S-adenosyl-L-homocysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pneumocystis carinii | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + 5alpha-cholesta-8,24-dien-3beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5alpha-cholest-8-en-3beta-ol | substrate binding, amino acid sequence | Pneumocystis carinii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
24-methylenelanosterol + S-adenosyl-L-methionine | preferred substrate | Pneumocystis carinii | pneumocysterol + S-adenosyl-L-homocysteine | - |
? | |
lanosterol + S-adenosyl-L-methionine | preferred substrate | Pneumocystis carinii | 24-methylene-24,25-dihydrolanosterol + S-adenosyl-L-homocysteine | - |
? | |
zymosterol + S-adenosyl-L-methionine | - |
Pneumocystis carinii | fecosterol + S-adenosyl-L-homocysteine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
recombinant enzyme | Pneumocystis carinii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | 45 | recombinant enzyme | Pneumocystis carinii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
recombinant enzyme | Pneumocystis carinii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | recombinant enzyme | Pneumocystis carinii |