Literature summary for 2.1.1.41 extracted from

Nes, W.D.; McCourt, B.S.; Zhou, W.X.; Ma, J.; Marshall, J.A.; Peek, L.A.; Brennan, M.
Overexpression, purification, and stereochemical studies of the recombinant (S)-adenosyl-L-methionine: DELTA24(25)-to DELTA24(28)-sterol methyl transferase enzyme from Saccharomyces cerevisiae (1998), Arch. Biochem. Biophys., 353, 297-311.

Search for more literature for 2.1.1.41

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Escherichia coli	Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
172000	-	gel filtration	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
zymosterol + S-adenosyl-L-methionine	Saccharomyces cerevisiae	-	fecosterol + S-adenosyl-L-homocysteine	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant wild-type from E. coli	Saccharomyces cerevisiae

Reaction

Reaction	Comment	Organism	Reaction ID
S-adenosyl-L-methionine + 5alpha-cholesta-8,24-dien-3beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5alpha-cholest-8-en-3beta-ol	mechanism	Saccharomyces cerevisiae	a
S-adenosyl-L-methionine + 5alpha-cholesta-8,24-dien-3beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5alpha-cholest-8-en-3beta-ol	substrate binding, amino acid sequence	Saccharomyces cerevisiae	a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	comparison recombinant and wild-type	Saccharomyces cerevisiae
0.0008	-	purified enzyme	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
zymosterol + S-adenosyl-L-methionine	-	Saccharomyces cerevisiae	fecosterol + S-adenosyl-L-homocysteine	-	?
zymosterol + S-adenosyl-L-methionine	regio- and stereospecific for substrates	Saccharomyces cerevisiae	fecosterol + S-adenosyl-L-homocysteine	-	?

Subunits

Subunits	Comment	Organism
More	cooperativity among the subunits	Saccharomyces cerevisiae
tetramer	-	Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
32	-	assay at	Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.01	-	Zymosterol	-	Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)