General Stability | Organism |
---|---|
no stabilization by MgCl2, CaCl2, DTT, reduced glutathione, phosphatidylcholine | Candida albicans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
desmosterol | competitive versus sterol and S-adenosyl-L-methionine | Candida albicans | |
desmosterol | competitive versus sterol and S-adenosyl-L-methionine | Candida tropicalis | |
S-adenosyl-L-homocysteine | - |
Candida albicans | |
Substrate analogues | dead-end inhibitors | Candida albicans | |
Zymosterol | competitive versus sterol and S-adenosyl-L-methionine | Candida albicans | |
Zymosterol | competitive versus sterol and S-adenosyl-L-methionine | Candida tropicalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.052 | - |
Zymosterol | - |
Candida tropicalis | |
0.071 | - |
Zymosterol | - |
Candida albicans | |
0.236 | - |
desmosterol | - |
Candida tropicalis | |
0.281 | - |
desmosterol | - |
Candida albicans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Candida albicans | - |
- |
microsome | - |
Candida tropicalis | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
150000 | - |
gel filtration | Candida albicans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
zymosterol + S-adenosyl-L-methionine | Candida albicans | - |
fecosterol + S-adenosyl-L-homocysteine | - |
? | |
zymosterol + S-adenosyl-L-methionine | Candida tropicalis | - |
fecosterol + S-adenosyl-L-homocysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | - |
- |
- |
Candida tropicalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Candida albicans |
- |
Candida tropicalis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + 5alpha-cholesta-8,24-dien-3beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5alpha-cholest-8-en-3beta-ol | mechanism | Candida albicans |
Storage Stability | Organism |
---|---|
-70°C, 1 month, less than 10% loss of activity | Candida albicans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
desmosterol + S-adenosyl-L-methionine | - |
Candida albicans | 24-methylenedesmosterol + S-adenosyl-L-homocysteine | - |
? | |
desmosterol + S-adenosyl-L-methionine | - |
Candida tropicalis | 24-methylenedesmosterol + S-adenosyl-L-homocysteine | - |
? | |
lanosterol + S-adenosyl-L-methionine | no reaction with | Candida albicans | 24-methylene-24,25-dihydrolanosterol + S-adenosyl-L-homocysteine | - |
? | |
zymosterol + S-adenosyl-L-methionine | - |
Candida albicans | fecosterol + S-adenosyl-L-homocysteine | - |
? | |
zymosterol + S-adenosyl-L-methionine | - |
Candida tropicalis | fecosterol + S-adenosyl-L-homocysteine | - |
? | |
zymosterol + S-adenosyl-L-methionine | zymosterol is 5alpha-cholesta-8,24-dien-3beta-ol | Candida albicans | fecosterol + S-adenosyl-L-homocysteine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Candida albicans |
30 | - |
assay at | Candida tropicalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Candida albicans |
7.5 | - |
assay at | Candida tropicalis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0045 | - |
Zymosterol | versus S-adenosyl-L-methionine | Candida tropicalis | |
0.009 | - |
desmosterol | versus S-adenosyl-L-methionine | Candida tropicalis | |
0.0193 | - |
desmosterol | versus S-adenosyl-L-methionine | Candida albicans | |
0.022 | - |
Zymosterol | versus sterol | Candida tropicalis | |
0.449 | - |
desmosterol | versus sterol | Candida tropicalis | |
0.489 | - |
desmosterol | versus sterol | Candida albicans |